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Practical Methods in Cardiovascular Research - S. Dhein

Practical Methods in Cardiovascular Research

By: S. Dhein (Editor), Friedrich-Wilhelm Mohr (Editor), Mario Delmar (Editor)

Hardcover Published: 1st February 2005
ISBN: 9783540407638
Number Of Pages: 1010

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Scientists working or planning to work in the field of cardiovascular research will welcome Practical Methods in Cardiovascular Research as the reference book they have long been waiting for. Not only general aspects of cardiovascular research are well presented, but also detailed descriptions of methods and protocols and practical examples. Written by leading scientists in their field, chapters cover classical methods such as the Langendorff heart or working heart models as well as numerous new techniques and methods. Newcomers and experienced researchers alike will benefit from the troubleshooting guide in each chapter, the extensive reference lists for advanced reading and the great practical experience of the authors. Practical Methods in Cardiovascular Research is therefore a long awaited "must have" for anybody with an interest in cardiovascular research.

In-Vivo Techniques
Models of Cardiovascular Diseases
Anaesthesia (Inhalative or i.v. Narcosis) of Laboratory Animalsp. 5
Extracorporeal Circulationp. 26
Open-chest Models of Acute Myocardial Ischemia and Reperfusionp. 37
Models of Chronic Ischemia and Infarctionp. 58
Models on Left Ventricular Hypertrophy (LVH)p. 72
Experimental Models of Heart Failurep. 83
In-Vivo Models of Arrhythmias: a Canine Model of Sudden Cardiac Deathp. 111
In-Vivo Models of Atrial Fibrillationp. 129
In-Vitro Techniques
Isolated Organs
The Langendorff Heartp. 155
The Working Heartp. 173
Isolated Papillary Musclesp. 190
Isolated Vesselsp. 198
Electrophysiological Techniques
Optical Techniques for the Recording of Action Potentialsp. 215
Recording Action Potentials Using Voltage-Sensitive Dyesp. 233
Epicardial Mapping in Isolated Hearts - Use in Safety Pharmacology, Analysis of Torsade de Pointes Arrhythmia and Ischemia-Reperfusion-Related Arrhythmiap. 256
Voltage-Clamp and Patch-Clamp Techniquesp. 272
L-Type Calcium Channel Recordingp. 324
Recording Cardiac Potassium Currents with the Whole-Cell Voltage Clamp Techniquep. 355
Recording the Pacemaker Current Ifp. 381
Recording Gap Junction Currentsp. 397
Recording Monophasic Action Potentialsp. 417
Heart on a Chip - Extracellular Multielectrode Recordings from Cardiac Myocytes in Vitrop. 432
Histological Techniques
Immunohistochemistry for Structural and Functional Analysis in Cardiovascular Researchp. 457
Classical Histological Staining Procedures in Cardiovascular Researchp. 485
Practical Use of in situ Hybridisation and RT in situ PCR in Cardiovascular Researchp. 500
Practical Use of Transmission Electron Microscopy for Analysis of Structures and Molecules in Cardiovascular Researchp. 525
DAF Technique for Real-Time NO Imaging in the Human Myocardiump. 546
Cell Culture Techniques
Isolation and Culture of Adult Ventricular Cardiomyocytesp. 557
Culture of Neonatal Cardiomyocytesp. 568
Culture of Embryoid Bodiesp. 577
Culturing and Differentiation of Embryonic and Adult Stem Cells for Heart Research and Transplantation Therapyp. 592
Preparation of Endothelial Cells from Micro- and Macrovascular Originp. 610
In Vitro Cultivation of Vascular Smooth Muscle Cellsp. 630
Engineering Heart Tissue for In Vitro and In Vivo Studiesp. 640
Biochemical and Analytical Techniques
High-Performance Liquid Chromatography (HPLC)p. 661
Protein Biochemistryp. 686
Receptor and Binding Studiesp. 723
Recording and Analysing Concentration-Response Curvesp. 784
Measurement of NO and Endothelial Function in Cardiovascular Researchp. 799
Biomechanical Stimulation of Vascular Cells In Vitrop. 813
Measurement of Function and Regulation of Adrenergic Receptorsp. 829
Measurement of Function and Regulation of Muscarinic Acetylcholine Receptorsp. 848
Cytometry in Cardiovascular Researchp. 863
Molecular Biology in Cardiovascular Research
Molecular Biology Techniques
Real-Time PCR Analysis of Cardiac Samplesp. 891
Use of cDNA Arrays to Explore Gene Expression in Genetically Manipulated Mice and Cell Linesp. 907
Using Antibody Arrays to Detect Protein-Protein Interactionsp. 916
Surface Plasmon Resonance as a Method to Study the Kinetics and Amplitude of Protein-Protein Bindingp. 936
How to Solve a Protein Structure by Nuclear Magnetic Resonance - The Connexin43 Carboxyl Terminal Domainp. 948
Managing a Transgenic Mouse Colony: A Guide for the Novicep. 959
Appendix
Colour Platesp. 979
Indexp. 997
Table of Contents provided by Ingram. All Rights Reserved.

ISBN: 9783540407638
ISBN-10: 3540407634
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 1010
Published: 1st February 2005
Publisher: Springer-Verlag Berlin and Heidelberg Gmbh & Co. Kg
Country of Publication: DE
Dimensions (cm): 23.5 x 15.5  x 3.81
Weight (kg): 1.71