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Practical Guide to Protein and Peptide Purification for Microsequencing - Paul T. Matsudaira

Practical Guide to Protein and Peptide Purification for Microsequencing

By: Paul T. Matsudaira (Editor)

Paperback Published: 20th May 1993
ISBN: 9780124802827
Number Of Pages: 184

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Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
Key Features
Highlights of the Second Edition:
* Mass spectrophotometric characterization of proteins and peptides
* N-terminal sequencing of proteins with blocked N termini
* Internal amino acid sequence analysis after protease digestion in-gel and on-blot
* Improved microscale peptide purification methods
* Computer analysis of protein sequences
* New protocols tested and refined through everyday use in authors' laboratories
* Updated reference chapter covering all aspects of protein microsequencing

Industry Reviews

Reviews of the First Edition: "Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world." --Cell

Introduction
Strategies for Obtaining Partial Amino Acid
Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein
Enzymatic Digestion of Proteins and HPLC Peptide Isolation
Purification of Proteins and Peptides By SDS-PAGE
Internal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on Nitrocellulose
Mass Spectrometric Strategies for the Structural Characterization of Proteins
Appendix
References
General Reference
Books on Protein Microsequencing Methodologies
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780124802827
ISBN-10: 0124802826
Audience: Professional
Format: Paperback
Language: English
Number Of Pages: 184
Published: 20th May 1993
Publisher: ACADEMIC PR INC
Country of Publication: US
Dimensions (cm): 23.09 x 16.1  x 1.14
Weight (kg): 0.29
Edition Number: 2
Edition Type: Revised

Earn 337 Qantas Points
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