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Postimplantation Mammalian Embryos : A Practical Approach - A. J. Copp

Postimplantation Mammalian Embryos

A Practical Approach

By: A. J. Copp (Editor), D. L. Cockroft (Editor)

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In recent years, the quest for greater understanding of postimplantation mammalian development has led research workers to devise techniques which overcome the problems of inaccessibility posed by embryos in the uterus, and to adapt analytic techniques such as in situ hybridization and antibody labelling to the unique requirements of mammalian embryonic material. This book fufils the need for detailed practical instruction in these methods and additionally provides a copiously illustrated account of the morphological stages of postimplantation development. Research workers in mammalian embryology will find this book invaluable both as a laboratory manual and as a reference work. It also complements Mammalian Development: A Practical Approach (ed. M. Monk), which covers pre-implantation techniques.

'stimulating and very clearly presented. I recommend it to those who are working, or considering working with this material. This volume is produced to the very high standare we are accustomed to in this series, with many excellent illustrations.'I. wilmut 'This third Practical Approach book in the series on mammalian development will be an invaluable resource ... enormous utility. We can no longer be satisfied with gene expression patterns and I hope granting committees will encourage investigation of biological questions which employ the living embryo. This book tells the investigator how to begin.' Karen Downs, University of Oxford, BioEssays, Vol. 14, No. 10 - October 1992

D.L. Cockroft: Dissection and culture of postimplantation embryos; K. Muneoka, C. Trevino, N. Wanek, & S.V. Bryant: Exo Utero surgery; V.E. Papaioannou: In Utero manipulations; M.H. Kaufman: Morphological stages of postimplantation embryonic development; N.A. Brown: Routine assessment of morphology and growth: scoring systems and measurements of size; M. Stanisstreet: Scanning electron microscopy and morphometry; M.M. Martins-Green: Transmission electron microscopy and immunolabelling for light and electron microscopy; D.G. Wilkinson, & J. Green: In Situ hybridization and the three-dimensional reconstruction of serial sections; J.P. Estibeiro, A.J. Copp, D.L. Cockroft, N.A. Brown, & D.O. Clarke: Extraction of macromolecules from embryonic material; M.F.W. Festing: Choice of an experimental animal and rodent genetics; C.E. Steele, & G.P. Copping: Teratogen testing; P.E. Mirkes, & A.G. Fantel: Maternal influences on development; S.J. Freeman: Functions of extraembryonic membranes; R.S.P. Beddington, & K.A. Lawson: Clonal analysis of cell lineages; A.J. Copp: Studying developmental mechanisms in intact embryos; P.P.L. Tam: Studying developmental in embryo fragments

ISBN: 9780199630899
ISBN-10: 0199630895
Series: Practical Approach Series
Audience: Professional
Format: Paperback
Language: English
Number Of Pages: 378
Published: 1st November 1990
Country of Publication: GB
Dimensions (cm): 23.6 x 15.7  x 2.3
Weight (kg): 0.74