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Plant Genomes Methods for Genetic and Physical Mapping - Jacques S. Beckmann

Plant Genomes Methods for Genetic and Physical Mapping

By: Jacques S. Beckmann (Editor), Thomas C. Osborn (Editor)

Hardcover Published: 22nd October 1992
ISBN: 9780792316305
Number Of Pages: 268

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The main application of genomic markers is the mapping, diagnosis, understanding, manipulation and eventual molecular cloning of loci of medical, biologic or economic interest. These achievements are likely to have a practical impact not only in basic science, e.g. in the elucidation of the basic processes involved in a particular pathway or in retracing the history of plant speciation, but also in breeding practice. In agriculture this translates into the exploitation of this knowledge for further genetic improvement since traits of agronomic interest are often polygenic. The elucidation of the genetic basis of polygenic inheritance and the subsequent efficient utilization of this knowledge in breeding schemes require the availability of an adequate network of genetic markers spanning the entire genome. The purpose of the present manual is to provide new investigators in this area with an introduction and a basic practical state-of-the-art description of how to proceed.

Simple plant DNA isolation proceduresp. 1
Methods of Southern blotting and hybridizationp. 15
Organelle DNA isolation and RFLP analysisp. 35
Detection of DNA sequence variation for genome analysisp. 55
Pulsed-field gel electrophoresisp. 71
Physical mapping by random clone fingerprint analysisp. 101
Construction and application of a YAC libraryp. 141
Linkage analysis in human geneticsp. 167
Statistical methodologies for mapping and analysis of quantitative trait locip. 181
Mapping quantitative trait loci using nonsimultaneous and simultaneous estimators and hypothesis testsp. 209
Epilogue: additional reflectionsp. 239
Indexp. 247
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780792316305
ISBN-10: 0792316304
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 268
Published: 22nd October 1992
Publisher: Kluwer Academic Publishers
Country of Publication: US
Dimensions (cm): 25.0 x 16.4  x 2.1
Weight (kg): 0.56

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