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Plant Development and Biotechnology - Robert N. Trigiano

Plant Development and Biotechnology

By: Robert N. Trigiano (Editor), Dennis J. Gray (Editor)

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Biotechnology revolutionized traditional plant breeding programs. This rapid change produced new discussions on techniques and opportunities for commerce, as well as a fear of the unknown. Plant Development and Biotechnology addresses the major issues of the field, with chapters on broad topics written by specialists. The book applies an informal style that addresses the major aspects of development and biotechnology with minimal references, without sacrificing information or accuracy. Divided into five primary parts, this volume explores how the field emerged from its early theoretical base to the technical discipline of today. It also covers progress being made with genetically engineered plants, providing a snapshot of the field's controversial present. Part III discusses methods for preparing media, creating solutions and dilutions, and accomplishing sterile culture work. It investigates common methods for visualizing and documenting studies, and quantifying responses of tissue culture in research. Part IV delivers the essential foundation of plant tissue culture, introducing the three types of commonly used culture regeneration systems. Part V integrates propagation techniques with other methodologies for the modification and manipulation of germplasm. Part VI concludes with special sections. Subjects include in vitro plant pathology, recent research into genetic and phenotypic variation, the mechanics of commercial plant production, and the importance of clean cultures and problems associated with maintaining in vitro cultures. The final chapter analyzes entrepreneurship in the field and outlines the do's and don'ts to consider when launching an enterprise.

"Many readers will be interested in the material on commercial production of micropropagated plantletsThis book is a pleasure to read, and fledgling plant biotechicians shoul dbe familiar with it. Summing up: Recommended." -CHOICE, March 2005, Vol. 42, No. 7

Introductionp. 3
History of plant tissue and cell culturep. 9
Getting started with tissue culture : media preparation, sterile technique, and laboratory equipmentp. 19
Historical techniquesp. 39
Photographic methods for plant cell and tissue culturep. 49
Elements of in vitro researchp. 55
A brief introduction to plant anatomyp. 73
Plant growth regulators in plant tissue culture and developmentp. 87
Software and databases as tools for analyzing nucleic acid and protein sequencesp. 101
Molecular approaches to the study of plant developmentp. 119
Shoot culture proceduresp. 145
Propagation from nonmeristematic tissues : organogenesisp. 159
Molecular aspects of in vitro shoot organogenesisp. 173
Propagation from nonmeristematic tissues : nonzygotic embryogenesisp. 187
Some developmental and molecular aspects of somatic embryogenesis (nonzygotic embryogenesis)p. 201
Use of protoplasts for plant improvementp. 213
Haploid culturesp. 225
Embryo rescuep. 235
Genetic engineering technologiesp. 241
Genetically modified plant controversies : sensational headlines versus sensible researchp. 251
Construction and use of a simple gene gun for particle bombardmentp. 265
A simple illumination system for visualizing green fluorescent proteinp. 273
Germplasm preservationp. 277
Valuable secondary products from in vitro culturep. 285
In vitro plant pathologyp. 293
Variation in tissue culturep. 301
Commercial laboratory productionp. 311
Indexing for plant pathogensp. 321
Entrepreneurship for biotechnology ventures : from bench to bagp. 333
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780849316142
ISBN-10: 0849316146
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 376
Published: 30th August 2004
Country of Publication: US
Dimensions (cm): 25.4 x 17.8  x 1.91
Weight (kg): 0.82
Edition Number: 1