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PCR 3 : PCR in Situ Hybridization: A Practical Approach - C. Simon Herrington


PCR in Situ Hybridization: A Practical Approach

By: C. Simon Herrington (Editor), John J. O'Leary (Editor)

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PCR 3: In Situ Hybridization is a unique and timely collection of well -tested protocols for the amplification of DNA and RNA in cells and ti ssues.

Industry Reviews

"The combination of the polymerase chain reaction (PCR) with in situ hybridization creates a powerful new technique that combines the best of both methods, enhances the sensitivity of in situ hybridization, and allows the morphological localization of the products of the PRC reaction. This volume. .
.provides detailed protocols for the use of polymerase chain reaction on tissues, interphase cells, and metaphase chromosomes. 4 Stars."--Doody's Journal
"Polymerase chain reaction (PCR) in situ hybridization (ISH) is a hybrid technique . . . The conventional use of ISH allows cellular detection and localization of DNA and RNA by hybridization with complementary nucleic acids probes and, thus, helps in understanding the disease process. PCR, on the
other hand, allows the detection of minute nucleic acids sequences, but it does not permit morphological correlation . . . The combination of both PCR and ISH has greatly enhanced the morphological localization of specific nucleic acids sequence in cells and tissues. . . . Overall, this book covers
all aspects of the technical details regarding PCR in situ hybridization and it will find wide use in those laboratories who perform or intend to perform in situ analyses of nucleic acids. The editors ought to be complimented for putting together the state of the art techniques in one book, which
may serve as a useful source in this technical field for a long time."--American Journal of Human Biology

1: Fixation of tissues for the polymerase chain reaction 2: In situ genetic analysis with selective ultraviolet radiation fractionation (SURF) 3: In situ hybridization 4: PCR in situ hybridization (PCR-ISH) 5: Reverse transcriptase in situ PCR for RNA detection 6: Primed in situ DNA synthesis (PRINS) 7: Application of in situ-PCR techniques to human tissues 8: Applications and modifications of PCR in situ hybridization 9: Automation of in situ amplification

ISBN: 9780199636327
ISBN-10: 019963632X
Series: Practical Approach Series
Audience: General
Format: Paperback
Language: English
Number Of Pages: 224
Published: 30th October 1997
Country of Publication: GB
Dimensions (cm): 23.42 x 15.65  x 1.22
Weight (kg): 0.32