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Oral Biology : Molecular Techniques and Applications - Gregory J. Seymour

Oral Biology

Molecular Techniques and Applications

By: Gregory J. Seymour (Editor), Mary P. Cullinan (Editor), Nicholas C. K. Heng (Editor)

Hardcover

Published: 31st August 2010
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It is generally recognized that the knowledge and research base that underpins dentistry lies in the biological and physical sciences. In this context, the major advances in these sciences over the past two decades have come through the application of molecular bi- ogy and nanotechnology. These advances are currently impacting on the diagnosis and treatment of a wide range of human diseases and it is essential that dental research, education, and practice keep pace with this rapidly advancing ?eld. As pointed out by Ford et al. (1): The de?nition of disease is also changing. Previously, disease was understood to be the presence of symptoms or of a particular phenotype. With increasing knowledge of the genetic basis of many diseases, this de?nition is changing to become the presence of a genotype conferring a pre-disposition to clinical symptoms or phenotype (Ford et al. (1)). This changing de?nition of disease means that today's undergraduate or graduate student in dentistry (and its related ?elds) must be in a position not only to acquire new knowledge in the future but also to be able to evaluate the information and apply it in a clinically relevant setting. This naturally positions oral biology as an integral part of any dentally related professional's repertoire of knowledge. There are as many topics in oral biology as there are the number of sites and micro- vironments within the oral cavity.

From the reviews:

"This book comprehensively reviews a wide range of techniques and protocols from diverse areas of oral biology, encompassing current research on microbes, host tissues and saliva. The book is obviously aimed at active researchers in the various fields, but because the research topics are so diverse, I would recommend purchase by institutes and departments ... ." (Adam Roberts, Microbiology Today, May, 2011)

Prefacep. v
Contributorsp. xi
Saliva Studies
Gene Therapy of Salivary Diseasesp. 3
Collection, Storage, and Processing of Saliva Samples for Downstream Molecular Applicationsp. 21
Proteomic Analysis of Saliva: 2D Gel Electrophoresis, LC-MS/MS, and Western Blottingp. 31
Transcriptomic Analyses of Salivap. 43
Oral Microbiology
The Oral Microbiota: General Overview, Taxonomy, and Nucleic Acid Techniquesp. 55
Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)p. 71
Protocols to Study the Physiology of Oral Biofilmsp. 87
Adhesion of Yeast and Bacteria to Oral Surfacesp. 103
Quantitative Analysis of Periodontal Pathogens by ELISA and Real-Time Polymerase Chain Reactionp. 125
Bacterial Viability Determination in a Dentinal Tubule Infection Model by Confocal Laser Scanning Microscopyp. 141
Characterization of Anti-competitor Activities Produced by Oral Bacteriap. 151
Natural Transformation of Oral Streptococcip. 167
Use of In Vivo-Induced Antigen Technology (IVIAT) to Identify Virulence Factors of Porphyromonas gingivalisp. 181
Oral Bacterial Genome Sequencing Using the High-Throughput Roche Genome Sequencer FLX Systemp. 197
Use of a Yeast-Based Membrane Protein Expression Technology to Overexpress Drug Resistance Efflux Pumpsp. 219
Cells and Tissues
Explant Culture of Embryonic Craniofacial Tissues: Analyzing Effects of Signaling Molecules on Gene Expressionp. 253
A Method to Isolate, Purify, and Characterize Human Periodontal Ligament Stem Cellsp. 269
Preclinical Methods for the Evaluation of Periodontal Regeneration In Vivop. 285
Proteomic Analysis of Dental Tissue Microsamplesp. 309
Immunological Techniques: ELISA, Flow Cytometry, and Immunohistochemistryp. 327
Analysis of Immune Responses to Purified Recombinant Antigens of Periodontal Pathogensp. 345
Single-Strand Conformation Polymorphism Analysis for the Diagnosis of T-Cell Clonality in Periodontal Diseasep. 359
Real-Time PCR Focused-Gene Array Profiling of Gingival and Periodontal Ligament Fibroblastsp. 373
The Use of Gene Arrays in Deciphering the Pathobiology of Periodontal Diseasesp. 385
Bioinformatics Techniques in Microarray Research: Applied Microarray Data Analysis Using R and SAS Softwarep. 395
Subject Indexp. 419
Table of Contents provided by Ingram. All Rights Reserved.

ISBN: 9781607618195
ISBN-10: 1607618192
Series: Methods in Molecular Biology
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 422
Published: 31st August 2010
Publisher: Humana Press Inc.
Country of Publication: US
Dimensions (cm): 25.4 x 17.8  x 3.18
Weight (kg): 1.11