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Nucleic Acid Hybridization : Introduction to Biotechniques S - M.L.M. Anderson

Nucleic Acid Hybridization

Introduction to Biotechniques S

Paperback Published: 1st November 1998
ISBN: 9781859960073
Number Of Pages: 260

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Simple practical guide which contains all relevant background information; ; Deals with the applications of nucleic acid hybridization in detail; ; Provides comprehensive trouble-shooting advice; ; This is an important practical guide which describes wha

Introduction to nucleic acid hybridizationp. 1
Types of hybridization and uses of each methodp. 11
Solution hybridization: reassociation of DNAp. 17
Solution hybridization: RNA:DNA hybridizationp. 35
Solution hybridization: reaction conditionsp. 49
Basic types of filter hybridizationp. 57
Hybridization strategy: long probesp. 65
Hybridization strategy: oligonucleotide probesp. 77
Choice of probep. 89
Basic techniques: binding of nucleic acid to filtersp. 97
Basic techniques: labeling of probesp. 115
Basic techniques: prehybridization, hybridization and washingp. 137
Basic techniques: detection of hybridsp. 147
Trouble shootingp. 167
Specific applications of techniquesp. 171
Current trendsp. 205
Appendicesp. 211
Appendix A: Glossaryp. 211
Solution hybridization equationsp. 215
Appendix C: Useful informationp. 219
Composition of stock solutionsp. 221
Suppliersp. 223
Indexp. 229
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9781859960073
ISBN-10: 1859960073
Series: Introduction to Biotechniques S
Audience: Professional
Format: Paperback
Language: English
Number Of Pages: 260
Published: 1st November 1998
Publisher: GARLAND PUB
Country of Publication: GB
Dimensions (cm): 23.39 x 15.6  x 1.4
Weight (kg): 0.37
Edition Number: 1

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