Nucleic acid hybridization techniques allow the detection of specific DNA or RNA sequences. This book is a clear and concise guide to the techniques used for preparing DNA and RNA for membrane hybridization. These include Southern blotting of DNA, northern blotting of RNA, dot/slot blotting, Benton-and-Davis screening of recombinant bacteriophage and Grunstein-Hogness screening of recombinant plasmids. It also discusses the pros and cons of using nitrocellulose filters and nylon membranes in these procedures. The book demystifies the laboratory manuals by explaining the rationale for each step in the published protocols and points out potential pitfalls with tips on how to avoid them.
The ideal support for newcomers - provides the answers to all the questions you want to ask.
|Slot and dot blotting|
|Choosing a membrane|
|Preparation of labelled probes|
|Hybridization of blots|
|Table of Contents provided by Publisher. All Rights Reserved.|
Number Of Pages: 124
Published: 15th June 1994
Publisher: Thomson West
Country of Publication: US
Dimensions (cm): 24.6 x 18.9 x 0.6
Weight (kg): 0.34
Edition Number: 1