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Nuclear Receptors : A Practical Approach - Didier Picard

Nuclear Receptors

A Practical Approach

By: Didier Picard (Editor)

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The steroid or nuclear receptor superfamily is an important group of transcription factors that is studied by a large and varied number of basic and clinical researchers. Nuclear Receptors: A Practical Approach provides these researchers an invaluable guide to do so. With an international list of contributors and examples of over 70 tried and true protocols, this book offers substantial insight into the growing family of biologically important receptors. Its chapters address a variety of factors, including the evolutionary biology of the superfamily and how to clone and characterize new receptors. Also addressed are topics such as identification of the ligands of novel receptors, the kinetic analysis of receptor interactions, and the functional characterization of coactivators using microinjection. Various sections throughout the book cover receptor phosphorylation, ligand regulated transcription, and hormone resistance syndromes. The book concludes with a description of yeast as a model system for looking at receptor function, and the final chapter shows how heterologous proteins can be regulated by fusion to the hormone binding domain of a receptor. Nuclear Receptors: A Practical Approach is a useful tool for any researcher studying members of the superfamily, and it is a must for all biomedical researchers.

"The editor more than meets his goal by providing technical information both for scientists interested in nuclear receptors and for others interested in the analysis of protein-mediated cellular processes. An exceptionally fine feature of this work is the layout of each specific protocol in an easy
to follow box format. These protocols range from the most simplistic, such as preparation of coverslips for microscopy to detailed descriptions of the assays used to analyze ligand binding by receptors. This would be a useful addition to any cell biology laboratory. The wealth of useful techniques
described should prove a useful starting point for anyone interested in pursuing the analysis of nuclear receptors as well as other cellular functions." -- Doody's

Preface Table of Contents List of Contributors 1: Evolutionary biology of the nuclear receptor superfamily 2: Initial characterization of new orphan receptors 3: Adopting orphans: finding ligands 4: Kinetic analysis of nuclear receptor interactions 5: Functional characterization of coactivators using mammalian cell microinjection 6: Analysis of steroid/nuclear receptor phosphorylation 7: Ligand- and cofactor-regulated transcription with chromatin templates 8: Hormone resistance syndromes 9: In Vitro assembly of steroid receptor - Hsp90 complexes 10: Yeast as a system for the study of nuclear receptor function 11: Regulation of heterologus proteins by fusion to a hormone binding domain List of non-standard Abbreviations List of suppliers Appendices

ISBN: 9780199637423
ISBN-10: 0199637423
Series: Practical Approach (Paperback)
Audience: General
Format: Paperback
Language: English
Number Of Pages: 304
Published: 8th July 1999
Country of Publication: GB
Dimensions (cm): 23.47 x 16.26  x 1.65
Weight (kg): 0.44
Edition Number: 2
Edition Type: Revised