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New Horizons in Nitrogen Fixation : Proceedings of the 9th International Congress on Nitrogen Fixation, Cancun, Mexico, December 6-12, 1992 - Rafael Palacios

New Horizons in Nitrogen Fixation

Proceedings of the 9th International Congress on Nitrogen Fixation, Cancun, Mexico, December 6-12, 1992

By: Rafael Palacios (Editor), Jaime Mora (Editor), William E. Newton (Editor)

Hardcover Published: 31st March 1993
ISBN: 9780792322078
Number Of Pages: 790

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In order to understand a process as complex as nitrogen fixation and to be in a position to manipulate it for the benefit of mankind, researchers are now working at the frontiers of science in many different areas: protein structure and function; catalytic mechanisms; electron transfer processes; regulatory circuits and environmental sensing; metabolic integration; chemical communication between organisms; differentiation; genome structure and function; microbial ecology; plant physiology; plant molecular biology; and agronomy. This volume represents a testimony to the advances in nitrogen fixation research that have been made and the contribution of these efforts to the solution of many other varied scientific problems. Limiting steps for future advances are analyzed and new horizons in nitrogen fixation research are proposed.

Overview Section
Introductory remarks at the overview sessionp. 3
Nitrogenases: Distribution, composition, structure and functionp. 5
Rhizobium and legume nodulation: A molecular dialoguep. 19
Plant function in nodulation and nitrogen fixation in legumesp. 31
Organization and regulation of nitrogen fixation genesp. 43
The role of respiration in symbiotic nitrogen fixationp. 55
Evolution since Knoxville: Were nitrogen-fixing organisms wise to inhabit land plants?p. 65
Biochemistry of Nitrogenases
An integrated, interdisciplinary approach to understanding the chemistry of nitrogenasesp. 79
Structures and functions of the nitrogenase proteinsp. 83
The crystal structure of the nitrogenase MoFe protein from Clostridium pasteurianump. 89
Kinetics and mechanism of nitrogenase - a role for 'P' centres in dinitrogen reductionp. 95
Nif gene products and their roles in nitrogen fixationp. 101
Intermolecular electron transfer and substrate reduction properties of MoFe proteins altered by site-specific amino acid substitutionp. 105
Use of Fe protein altered at specific amino acid positions to probe its function in nitrogenase catalysisp. 111
Metal regulation of nitrogenases and molybdenum transport in Azotobacter vinelandiip. 117
Regulatory role of molecular chaperones in nitrogenase biogenesisp. 123
Bacteria-Plant Interaction
Nod factors from Rhizobium fredii USDA257p. 157
The sulfate group on the reducing end protects nod signals of R. meliloti against hydrolysis by Medicago chitinasesp. 159
The function of the rhizobial nodABC and nodFEL operons in the biosynthesis of lipo-oligosaccharidesp. 165
Chemical signaling of Rhizobium nodulating beanp. 171
Bradyrhizobium japonicum strains produce multiple 2-0-methylfucose-containing lipo-oligosaccharide nodulation signalsp. 177
Nod genes and nod factors of Rhizobium species NGR234p. 183
Nod factor production by Azorhizobium caulinodans strain ORS571p. 191
Signals released from alfalfa regulate microbial activities in the rhizospherep. 197
Exopolysaccharides and Rhizobium meliloti-alfalfa interactionsp. 203
The role of Rhizobium meliloti surface polysaccharides in nodule developmentp. 207
Rhizobium lequminosarum CE3 lipopolysaccharide structure required in symbiosis with beanp. 213
Bacteria-Plant Interaction
Pea nod[superscript -] mutants and nod factor perceptionp. 267
Biogenesis of peribacteroid membrane (PMB) forming a subcellular compartment essential for symbiotic nitrogen fixationp. 269
Characterization of GmENOD40, a gene expressed in soybean nodule primordiap. 275
Expression of the Medicago truncatula ENOD12 gene in response to R. meliloti nod factors and during spontaneous nodulation in transgenic alfalfap. 285
Molecular interactions during nodule developmentp. 291
Nodule morphogenesis in the absence of Rhizobiump. 297
Ethylene and nodulationp. 303
Differential gene expression in the development of actinorhizal root nodulesp. 309
A cyanobacterial-plant endosymbiosis: Aspects of its establishmentp. 315
Potential targets and pathways for Rhizobium's mitogenic stimulusp. 321
Regulation and Genetics of Nitrogen Fixation
Molecular analysis of components responsible for protection of Azotobacter nitrogenase from oxygen damagep. 383
The regulation of oxygen and its role in regulating nodule metabolismp. 393
In vivo and in vitro oxygen control of nitrogen fixation gene expression in Rhizobium melilotip. 399
Oxygen sensing by the Rhizobium meliloti two-component regulatory system, FixLJp. 405
Two oxygen-responsive regulatory cascades control nitrogen fixation genes in Bradyrhizobium japonicump. 411
Heterocyst differentiation and nitrogen fixation in the cyanobacterium Anabaenap. 417
Regulation of nifA synthesis and activity in Azospirillum brasilense Sp7p. 423
The nifL gene of Azotobacter vinelandii: Novel features of sequence, expression and mutant phenotypesp. 429
A maltose-binding protein-nifA (MBP-NIFA) fusion activates transcription from the Klebsiella pneumoniae nifH promoter in a purified system: Stimulation by IHFp. 435
Carbon and Nitrogen Metabolism
Carbon and nitrogen metabolism in Rhizobiump. 513
Succinate metabolism in Rhizobium melilotip. 517
Diversion of carbon to polysaccharide synthesis by Bradyrhizobium japonicum and B. elkanii bacteroidsp. 523
Molecular control of alfalfa nodule NADH-glutamate synthase: A key for nitrogen assimilationp. 529
Genome Structure, Evolution and Ecology
Rearrangement of nif operons in cyanobacterial heterocystsp. 575
Gene amplification in Rhizobiump. 581
Molecular phylogeny of rhizobia and their relativesp. 587
Environmental and Applied Aspects
The prospects of increasing the input of biological nitrogen fixation in agriculturep. 667
Endophytic diazotrophs in sugar cane, cereals and tuber plantsp. 671
Prospects and problems of biological nitrogen fixation in rice production: A critical assessmentp. 677
Diversity of photosynthetic Bradyrhizobium strains from stem nodules of Aeschynomene speciesp. 683
Capacities of Azoarcus sp., a new genus of grass-associated diazotrophsp. 691
Field testing of genetically engineered rhizobiap. 695
Workshop Reports
Mechanism of nitrogenase catalysisp. 759
Extension of nitrogen fixation to other cropsp. 765
Regeneration and transformation of legumesp. 769
Competitiveness of Rhizobium strains for nodule occupancyp. 773
Report from the Taxonomy Meeting, Subcommittee on Agrobacterium and Rhizobiump. 777
Author Indexp. 779
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780792322078
ISBN-10: 079232207X
Series: Current Plant Science and Biotechnology in Agriculture
Audience: General
Format: Hardcover
Language: English
Number Of Pages: 790
Published: 31st March 1993
Country of Publication: NL
Dimensions (cm): 23.39 x 15.6  x 4.29
Weight (kg): 1.3

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