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Neural Transplantation Methods : Neuromethods - Stephen B. Dunnett

Neural Transplantation Methods

Neuromethods

By: Stephen B. Dunnett (Editor), Alan A. Boulton (Editor), Glen B. Baker (Editor)

Hardcover Published: 20th October 1999
ISBN: 9780896037939
Number Of Pages: 556

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we might seek alternative sources of donor tissues. Genetic en- neering, expansion of precursor cells, generation of immortalized cell lines, and transplantation between species are all under active investigation. Although significant difficulties remain for each of these alternatives, the problems appear soluble and relevant knowledge is expanding rapidly. As we enter the twenty-first century, the place of neural tra- plantation in experimental neuroscience is continuing to evolve. Rather than being a topic in its own right, neural transplantation increasingly serves as just another technique in the researcher's armory--alongside lesions, pharmaceuticals, gene transfer, and a variety of other techniques--for the experimental manipulation of brain structure and function. This is particularly true for studies of degeneration, plasticity, regeneration, and recovery of function in the nervous system, topics of increasing importance as experimental neurobiology is required to serve the higher needs of neurological and mental health in aging societies. Within this evolving context, Neural Transplantation Methods seeks to serve a particular need: to provide experimental neu- scientists with a source book of information to enable them to select and adapt transplantation techniques to their own expe- mental programs. All authors have been asked to address prac- cal issues, to enable the reader to assess what is available, what are the alternatives, what are the practical issues to be resolved in applying a particular protocol and getting it to work reliably in their unique experimental context.

Industry Reviews

"...a book with so much information is very valuable for neuroscientists."- Clinical Neurology and Neurosurgery

"Neural Transplantation Methods seeks to serve a particular need: to provide experimental neuroscientists with a source book of information to enable them to select and adapt transplantation techniques to their own experimental programs....Neural Transplantation Methods is a very interesting, up-to-date, and complete book on the technical and methodological issues in relation with experimental neural transplantation. It is a perfect source book of information for those who want to include the transplantation strategy in their experimental program." - Acta Neurologica Belgica

Preface to the Seriesp. v
Prefacep. vii
Contributorsp. xiii
Sources of Cells for Transplantation
Dissecting Embryonic Neural Tissues for Transplantationp. 3
Pregraft Tissue Storage Methods for Intracerebral Transplantationp. 27
Neural Stem Cells for Transplantationp. 41
Immortalized Cellsp. 55
Cells from Testis for Transplantation in the CNSp. 73
Engineered Cells and Ex Vivo Gene Transferp. 89
Intracerebral Gene Transfer Using Viral Vectorsp. 103
Methods of Implantation
Basic Transplantation Methods in Rodent Brainp. 133
Intraocular Graftingp. 149
Neural Transplantation in the Developing CNSp. 169
Preparation of Cell Suspensions for Transplantationp. 195
Microtransplantation of Nigral Dopamine Neurons: A Step-By-Step Recipep. 207
Application of Polymer-Encapsulated Cell Therapy for CNS Diseasesp. 233
Neural Transplantation in Parkinsonian Primatesp. 279
Transplanting Myelinogenic Cells into the CNSp. 305
Labeling and Identifying Grafted Cellsp. 319
Factors in Graft Survival and Function
Neovascularization and the Blood-Brain Barrier in Neural Transplantationp. 365
Trophic Influences on Neural Tissue Transplants: Delivery Methods and Co-graft Interactionp. 385
Neuroprotective Strategies in Neural Graftingp. 411
Death and Survival in CNS Graftingp. 441
Immunology: Mechanisms of Rejectionp. 461
Immunoprotection of Cell and Tissue Implants in the CNSp. 477
Immunobiology of Neural Xenotransplantationp. 503
Indexp. 543
Table of Contents provided by Syndetics. All Rights Reserved.

ISBN: 9780896037939
ISBN-10: 0896037932
Series: Neuromethods
Audience: General
Format: Hardcover
Language: English
Number Of Pages: 556
Published: 20th October 1999
Publisher: SPRINGER VERLAG GMBH
Country of Publication: US
Dimensions (cm): 23.7 x 16.05  x 3.79
Weight (kg): 1.23

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