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Mycobacterium Tuberculosis Protocols : Methods in Molecular Biology - Tanya Parish

Mycobacterium Tuberculosis Protocols

Methods in Molecular Biology

By: Tanya Parish (Editor), Neil G. Stoker (Editor)

Hardcover Published: 19th May 2001
ISBN: 9780896037762
Number Of Pages: 406

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Leading investigators with extensive practical knowledge and experience describe their best methods for studying the tuberculosis pathogen. Packed with step-by-step instructions to ensure successful results, these methods range from basic handling techniques to the application of functional genomics. Highlights include methods for the basic safety and culture of M. tuberculosis, fractionation of the bacterium (nucleic acids, lipids, culture filtrate, and capsule), the analysis of gene expression (start site mapping, real-time PCR microarrays, and proteomics), the growth of the bacterium in macrophages and low oxygen, cytological analysis of the bacteria, and diagnostics. Highly practical and accessible, Mycobacterium tuberculosis Protocols utilizes advanced functional genomics and mutagenesis methodologies to provide both experimental and clinical investigators all the powerful techniques needed to illuminate the molecular biology of tuberculosis and its interactions with host cells, and so drive work on the wide variety of emerging therapeutic opportunities.

Industry Reviews

...a very useful book for the mycobacteria research community. It is a specialist's cookbook, perfect if you want to establish a new method that is outside your scope of expertise. Individual chapters are clearly divided into introduction, materials, methods and an extensive list of notes. -- International Journal of Medical Microbiology

"For those engaged in mycobacterial research (and possibly other unrelated genera) which involves genetics, biochemistry and immunology, this textbook is essential and, moreover, I would recommend it as reading material for students undertaking molecular biology experimentation." -Today's Life Science

Prefacep. v
Contributorsp. xi
Plasmid Vectorsp. 1
Isolation of DNA from Mycobacterium tuberculosisp. 19
Extraction of RNA from Intracellular Mycobacterium tuberculosis: Methods, Considerations, and Applicationsp. 31
Transposon Mutagenesis in Mycobacteria Using Conditionally Replicating Mycobacteriophagesp. 43
Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacBp. 59
Gene Replacement Using Pretreated DNAp. 77
Gene Replacement in Mycobacterium tuberculosis and Mycobacterium bovis BCG Using rpsL+ as a Dominant Negative Selectable Markerp. 93
Transcription Start-Site Mappingp. 105
Fluorescence and Brightfield Cytology of Live M. tuberculosis Cellsp. 125
Phage Replication Technology for Diagnosis and Drug Susceptibility Testingp. 145
Detection of Mutations in Mycobacterium tuberculosis by a Dot Blot Hybridization Strategyp. 153
Restriction Fragment Length Polymorphism Typing of Mycobacteriap. 165
Preparation of Culture Filtrate Proteins from Mycobacterium tuberculosisp. 205
Analysis of the Capsule of Mycobacterium tuberculosisp. 217
Analysis of the Lipids of Mycobacterium tuberculosisp. 229
In Vitro Model of Hypoxically Induced Nonreplicating Persistence of Mycobacterium tuberculosisp. 247
Macrophage Virulence Assaysp. 271
Analysis of Mycobacterium-Infected Macrophages by Immunoelectron Microscopy and Cell Fractionationp. 281
Real-Time PCR Using Molecular Beacons: A New Tool to Identify Point Mutations and to Analyze Gene Expression in Mycobacterium tuberculosisp. 295
Electronic Access to Mycobacterium tuberculosis Sequence Datap. 311
Proteomicsp. 315
Functional Genomics of Mycobacterium tuberculosis Using DNA Microarraysp. 335
Storage of Mycobacterial Strainsp. 359
Safety in the Laboratoryp. 367
Indexp. 385
Table of Contents provided by Syndetics. All Rights Reserved.

ISBN: 9780896037762
ISBN-10: 0896037762
Series: Methods in Molecular Biology
Audience: General
Format: Hardcover
Language: English
Number Of Pages: 406
Published: 19th May 2001
Publisher: Humana Press Inc.
Country of Publication: US
Dimensions (cm): 23.65 x 16.1  x 3.12
Weight (kg): 0.8

Earn 542 Qantas Points
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