Although most often considered in terms of the generation of action potentials, there are several other electrical, metabolic and ionic correlates of neuronal activity. This book draws together, in a single volume, ten different facets of the activity of nerve cells. There are chapters on the monitoring of extra-cellular unit activity, intracellular voltage and current clamping, patch clamping, video-imaging, in vivo voltammetry, intracerebral dialysis, antibody microprobes, 2-deoxyglucose uptake, intracellular fluorescence and ion-selective electrodes. The techniques described range from the well-established to the experimental. In each case the chapters include up-to-date information, methodological tips and detailed protocols allowing the reader to follow each method in a stepwise fashion. The wide range of techniques in the book should be of interest to neuroscientists, whether concerned with traditional or novel indices on neuronal function.
'This book is part of the excellent 'Practical Approach' series. With its well-laid-out format, protocols and source references, the whole series and this book in particular is of use to undergraduates, graduates, senior researchers and even professors.'
Jon Robbins, University College, London, TiPS, November 1992 (Vol. 13)
Section I: The electrical activity of nerve tissue: J. Millar: Extracellular single and multiple unit recording with microelectrodes; E.M. Silinsky: Intracellular recording methods for neurones; N.B. Standen & P. Stanfield: Patch clamp techniques for single channel and whole cell recording; M.M. Haglung & G.G. Blasdel: Video imaging of neuronal activity using voltage-sensitive dyes; Section II: Neurotransmitter release and metabolism: J.A.
Stamford, F. Crespi & C.A. Marsden: In vivo voltammetric methods for monitoring monoamine release and metabolism;T. Sharp & T. Zetterstrom: In vivo measurement of neurotransmitter release and metabolism using brain
microdialysis; A.W. Duggan: Preparation, use and analysis of antibody microprobes; Section III: Metabolic and ionic indices of nerve cell activity: L. Sokoloff, C. Kennedy & C.B. Smith: Metabolic mapping of local neuronal activity; M.R. Duchen: Fluorescence-monitoring cellular chemistry in vivo; E. Sykova: Ion selective microelectrodes.