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Molecular Interactions in Bioseparations : Physics of Atoms and Molecules - That T. Ngo

Molecular Interactions in Bioseparations

Physics of Atoms and Molecules

By: That T. Ngo (Editor)

Hardcover Published: 31st December 1993
ISBN: 9780306444357
Number Of Pages: 570

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Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purifica­ tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chromatography and includes a chapter describing an interesting new technique called "slalom chromatography" for DNA fractionations. Affinity chromatography using molecules of biological origin as the affinity ligand is covered in Part II. Part III describes the use of a special class of biomolecules, antibodies, as affinity ligands. Affinity chromatography with biomimetic ligands is discussed in Part IV. Newer concepts and their applications in bioseparation are presented in Part V. Part VI covers affinity-related techniques such as affinity-based extracorporeal shunts, affinity electrophoresis, affinity precipitation, and affinity extraction. I would like to express my sincere thanks to all the authors, who are recognized experts in their respective fields, for their cooperation and contributions. I thank the editorial staff of Plenum Press for their professionalism, and Mary Phillips Born, Senior Editor, for her encouragement. The support of my family (Ping and Peilin) made it possible to complete editing this book. That T.

Affinity Chromatography: An Overviewp. 3
Weak-Affinity Chromatographyp. 15
Affinity Chromatography on Inorganic Support Materialsp. 27
2-Fluoro-1-methylpyridinium (FMP) Salt-Activated Gels: Properties and Uses in Affinity Chromatography and Enzyme Immobilization for Analytical Applicationsp. 49
Slalom Chromatography: A Size-Dependent Separation Method for DNA Molecules Based on a Hydrodynamic Principlep. 69
Applications of Bacterial Immunoglobulin-Binding Proteins to the Purification of Immunoglobulinsp. 91
Affinity Chromatography of Oligosaccharides and Glycopeptides with Immobilized Lectinsp. 113
Selective Isolation of C-Terminal Peptides by Affinity Chromatographyp. 127
Receptor-Affinity Chromatography (RAC)p. 139
Immobilized Artificial Membrane Chromatography: Prediction of Drug Transport across Biological Barriersp. 151
Affinity Chromatography Using Immobilized Antisense-Family Peptidesp. 169
Chromatographic Resolution of Chiral Compounds by Means of Immobilized Proteinsp. 179
Chromatography with Cyclodextrin-Based Stationary Phasesp. 189
Suitable Antibodies as Ligands in Affinity Chromatography of Biomoleculesp. 205
Immunoaffinity Purification of Organellesp. 213
Immunoaffinity Separation of Cells Using Monosized Magnetic Polymer Beadsp. 229
Affinity Chromatography with Immobilized Dyesp. 247
Pseudo-Biospecific Affinity Ligand Chromatography: The Case of Immobilized Histidine as a Universal Ligandp. 257
Synthetic Protein Surface Domains as Bioactive Stationary Phases: Metal Ion-Dependent Macromolecular Recognition and Biospecific Metal Ion Transferp. 277
Affinity Chromatography with Immobilized Benzeneboronatesp. 313
Affinity Chromatographic Removal of Pyrogensp. 323
Molecular Interactions in Hydrophobic Chromatographyp. 333
Biorecognition in Molecularly Imprinted Polymers: Concept, Chemistry, and Applicationp. 363
Bioseparation and Catalysis in Molecularly Imprinted Polymersp. 383
Use of Heterobifunctional Ligands in Affinity Chromatographic Processesp. 395
Covalent Chromatographyp. 403
Aza-Arenophilic Interaction: Novel Mode of Protein Adsorption and Applications in Immunoglobulin Purificationp. 415
Model Systems Employing Affinity Chromatography for Extraction of Toxic Substances Directly from Whole Bloodp. 433
Applications of Affinity Binding to the Development of Heparin Removing and Sensing Devicesp. 451
Affinity Precipitationp. 469
Affinity Separation of Nucleic Acids on Monosized Magnetic Beadsp. 479
Affinity Ultrafiltration for Protein Purificationp. 487
Affinity Partitioning of Biomolecules in Aqueous Two-Phase Systemsp. 499
Affinity Electrophoresis of Macromolecules: General Principles and Their Application to Nucleic Acidsp. 511
Reversed Micelles for Protein Purificationp. 531
Indexp. 545
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780306444357
ISBN-10: 0306444356
Series: Physics of Atoms and Molecules
Audience: General
Format: Hardcover
Language: English
Number Of Pages: 570
Published: 31st December 1993
Publisher: Springer Science+Business Media
Country of Publication: US
Dimensions (cm): 25.4 x 17.78  x 3.18
Weight (kg): 1.23

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