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Molecular Histochemical Techniques : Springer Lab Manuals - Takehiko Koji

Molecular Histochemical Techniques

Springer Lab Manuals

By: Takehiko Koji (Editor)

Hardcover

Published: 17th April 2000
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Molecular Histochemical Techniques presents detailed protocols for analyzing DNA strand breaks, specific RNA/DNA expressions, and DNA binding proteins such as transcription factors at the individual cell level. In situ nick translation and TUNEL are described in detail, along with radioactive and nonradioactive methods for in situ hybridization. Of special significance is the description of Southwestern histochemistry, a new development in molecular histochemistry, which makes it possible to analyze the expression of transcription regulatory factors in individual cells. This volume provides an overview of the current status of molecular histochemistry along with practical how-to-do-it details. Methodology is set forth step-by-step in easy-to-follow language by leading scientists who are working in the field today. The book serves as both a rich source of the latest information and a practical lab manual.

Introduction to the Detection of DNA Strand Breaksp. 3
In Situ Nick Translationp. 13
In Situ Nick End-Labeling: Light Microscopicalp. 19
In Situ Nick End-Labeling: Electron Microscopicalp. 31
Introduction to the Detection of Specific DNA and RNA Sequencesp. 49
In Situ Hybridization for DNA: Fluorescent Probep. 65
Subprotocol 1: Fluorescence In Situ Hybridization (FISH)p. 68
Subprotocol 2: Protocol for Rapid Procedurep. 88
Subprotocol 3: Protocol for Codenaturationp. 90
Subprotocol 4: Comparative Genomic Hybridization (CGH)p. 92
In Situ Hybridization for RNA: Radioactive DNA Probep. 100
In Situ Hybridization for RNA: Radioactive RNA Probe - A Protocol for Practice With a Kitp. 115
In Situ Hybridization for RNA: Nonradioactive probe: ds cDNA Probep. 128
In Situ Hybridization for RNA: Nonradioactive Probe: ss cDNA Probep. 139
In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: T-T Dimerp. 154
In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: Digoxigenin (I)p. 172
In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: Digoxigenin (II)p. 182
In Situ Hybridization for RNA: Nonradioactive Probe: RNA Probep. 196
Electron Microscopic in Situ Hybridization and its Combination With Immunohistochemistryp. 204
Whole Mount in Situ Hybridization for mRNA detection in Chick Embryosp. 222
Introduction to the Detection of Transcription Regulatory Proteinsp. 237
Southwestern Histochemistryp. 244
Subject Indexp. 253
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9784431702313
ISBN-10: 4431702318
Series: Springer Lab Manuals
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 263
Published: 17th April 2000
Publisher: Springer Verlag, Japan
Country of Publication: JP
Dimensions (cm): 23.5 x 15.5  x 1.27
Weight (kg): 0.7