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Molecular Genetics of Yeast : A Practical Approach - John R. Johnston

Molecular Genetics of Yeast

A Practical Approach

By: John R. Johnston (Editor)

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The book covers comprehensively all current experimental procedures used in the research of the genetics and molecular biology of the yeast Saccharomyces cerevisiae. Featuring detailed protocols and practical tips, it guarantees easy access to a wide range of specialized topics within this rapidly advancing field. Internationally-recognized experts present all methods currently in use, discussing topics such as DNA isolulation, cloning and expression vectors, cosmid cloning, construction and use of cDNA libraries, plasmid shuffling and mutant isolulation. Chapters on Ty insertional mutagenesis, high efficiency transformation, cell-free translation of mRNAs, Ty virus-like particles, and applications to industrial strains of yeast are also included. Researchers in the fields of molecular biology, genetics, and biochemistry working with this yeast, as well as professionals of the biotechnology industry will refer to this practical reference frequently.

The distinguished collection of authors are often pioneers in their subject area and so the guidance is both authoritative and helpful. Overall this is a useful addition to the series, with ready access to standard lab protocols. * Alan Wheals, Society for General Microbiology, November 1996 *

1: Procedures for isolating yeast DNA for different purposes 2: Construction of cloning and expression vectors 3: Cosmid cloning of yeast DNA 4: The construction and use of cDNA libraries for genetic selections 5: Pulsed field gel electrophoresis 6: Plasmid shuffling and mutant isolation 7: Ty insertional mutagenesis 8: High efficiency transformation with lithium acetate 9: Measurement of transcription 10: Measurement of mRNA stability 11: Production of foreign proteins at high level 12: Cell-free translation of natural and synthetic mRNAs 13: Virus-like particles: Ty retrotransposons 14: The K1 killer toxin: molecular and genetic applications to secretion and cell surface assembly 15: Immuno-electron microscopy 16: Industrial Saccharomyces yeasts

ISBN: 9780199634293
ISBN-10: 0199634297
Series: Molecular Genetics of Yeast
Audience: General
Format: Paperback
Language: English
Number Of Pages: 312
Published: 28th February 2002
Country of Publication: GB
Dimensions (cm): 23.39 x 15.6  x 1.6
Weight (kg): 0.42