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Molecular Diagnostics - George Patrinos

Hardcover Published: 6th June 2005
ISBN: 9780125466615
Number Of Pages: 488

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Molecular Diagnostics covers current molecular biological techniques used to identify the underlying molecular defects in inherited disease. Although an increasing number of laboratories, both academic and private are moving in that direction, there are only a few books in the existing literature, and they deal only partly with diagnosis at the molecular level. Each chapter includes the principle and a brief description of the technique, followed by exmples from the authors' own epertise. Contributors are well-known experts in their field, and derive from a variety of disciplines, to ensure breadth and depth of coverage.
* Examines widely used molecular biology techniques to screen for genetic defects causing inherited disorders
* Includes state-of-the-art techniques for the detection of the underlying genetic heterogeneity leading to inherited disorders
* Identification of genetically modified organisms (GMO's)
* Forensic analysis and every-day issues in a diagnostic laboratory
* Discusses ethics, genetic counselling and quality management

"...the book is uniform in style. The text is free of jargon and is well written in an easy to understand style. ...This book is a great resource for experienced readers, giving them a wide overview of the most up-to-date methods. It is also a great book for students and medical professionals who want to learn about the molecular tools used in diagnostics. ...a great teaching aid for courses involving molecular biology, genetics and diagnostic techniques." - AUSTRALIAN BIOCHEMIST (April 2006) "...the book will be useful to investigators in the diagnostic laboratory and to scientists generally who want a summary of current methods. ...Inclusion of ethical and safety considerations (as well as a section on patents) is a distinct plus, not often seen. ...this is a well organized and edited contribution." - DOODY'S (October 2005)

Molecular diagnostics : past, present, and futurep. 1
Allele-specific mutation detection by PCR-ARMS and PCR-ASOp. 15
Competitive oligoprimingp. 23
Oligonucleotide ligation assays for the diagnosis of inherited diseasesp. 31
Enzymatic and chemical cleavage methods to identify genetic variationp. 41
Mutation detection by single-strand conformation polymorphism and heteroduples analysisp. 55
Capillary electrophoresisp. 67
Temperature and denaturing gradient gel electrophoresisp. 83
Two-dimensional gene scanningp. 97
Real-time polymerase chain reactionp. 109
Pyrosequencingp. 127
Molecular cytogenetics in molecular diagnosticsp. 139
Detection of genomic duplications and deletionsp. 159
Analysis of human splicing defects using hybrid minigenesp. 171
DNA microarrays and genetic testingp. 185
Human genome microarray in biomedical applicationsp. 201
Use of high throughput mass spectrographic methods to identify disease processesp. 211
The application of proteomics to disease diagnosticsp. 223
Identification of genetically modified organismsp. 235
Pharmacogenetics and pharmacogenomics : impact on drug discovery and developmentp. 249
Molecular diagnostic applications in forensic sciencep. 267
Molecular diagnostics and comparative genomics in clinical microbiologyp. 281
Preimplantation genetic diagnosisp. 297
Genetic monitoring of laboratory animalsp. 311
The use of locus-specific databases in molecular diagnosticsp. 319
Safety analysis in retroviral gene therapy : identifying virus integration sites in gene-modified cellsp. 327
Automated DNA hybridization and detectionp. 337
The use of microelectronic-based techniques in molecular diagnostic assaysp. 349
Miniturization technologies for molecular diagnosticsp. 365
Human gene patents and genetic testingp. 391
Genetic counselling and ethics in molecular diagnosticsp. 399
Genetic testing and psychologyp. 409
Safety in biomedical and other laboratoriesp. 421
Quality management in the laboratoryp. 429
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780125466615
ISBN-10: 0125466617
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 488
Published: 6th June 2005
Publisher: Elsevier Science Publishing Co Inc
Country of Publication: US
Dimensions (cm): 27.31 x 21.59  x 2.54
Weight (kg): 1.38