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Molecular Diagnosis of Cancer : Methods and Protocols - Joseph E. Roulston

Molecular Diagnosis of Cancer

Methods and Protocols

By: Joseph E. Roulston (Editor), John M. S. Bartlett (Editor)

Hardcover Published: 15th March 2004
ISBN: 9781588291608
Number Of Pages: 393

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We are currently experiencing a fundamental shift in the way in which we approach the characterization of cancer. Never before has the make up of cancer tissues and individual cells been so exhaustively researched and char- terized. We are now capable of producing molecular "fingerprints" that ch- acterize the expression of all known and unknown genes within tumors and their surrounding tissues. More than 30,000 different genes may be measured in each patient's tumor in a single experiment. Simultaneously, novel therapies that exploit the molecular roadmap have been developed and are now being offered to patients. These novel agents, such as Glivec, Herceptin, Iressa, and others, specifically target individual genes within tumors and can produce d- matic responses in some patients. These drugs are only the forerunners of a coming tidal wave of novel therapeutics that individually target specific m- ecules within cancer cells-more than 300 such agents are currently in phase I or II clinical trials. This is an exciting time for cancer specialists and patients alike. However, if we have learned anything from the past 50 or more years of research into cancer, it is that Lord Beaverbrook, in founding the British national health service in the 1950s, was frighteningly prescient when he defined the primary goal of health care to be "Diagnosis, Diagnosis, Diag- sis. " Now, more than ever, it is essential that appropriate diagnostic methods and approaches are applied to the selection of patients for treatment.

"...concise and uniformly well written...contains a large compilation of applications of nucleic acid technologies that are in use or may be in use in the near future to diagnose and aid in the management of malignant diseases." - Doody's Health Sciences Book Review Journal

Review of the First Edition
The techniques described here are standard laboratory lore;the added value are the notes and tips on tricky details andoften overlooked precautions....Laboratory scientists...will get their money's worth. -Modern Pathology

"....a diverse collection of readily reproducible methods...the protocols presented follow step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls." - Tumori

From the reviews of the second edition:

"Dramatic advances in molecular biology over the past decades have led to an unprecedented understanding of cancer at a very detailed molecular level. ... tips on troubleshooting are presented in each chapter, written by experts in the field. Given the amount of detail incorporated in each protocol, these should be readily reproducible and enable laboratories with no prior experience in a specific technique to master it and be able to use todays most powerful techniques in the molecular diagnosis of cancer." (Janis Racevskis, Medical Oncology, Vol. 22 (3), 2004)

Prefacep. v
Contributorsp. ix
Prognostic and Predictive Factorsp. 1
Assessment of Predictive Values of Tumor Markersp. 13
Quality Assurance of Predictive Markers in Breast Cancerp. 29
Extraction of Nucleic Acid Templatesp. 59
Microdissection and Extraction of DNA From Archival Tissuep. 71
Fluorescence In Situ Hybridization: Technical Overviewp. 77
HER2 FISH in Breast Cancerp. 89
Fluorescence In Situ Hybridization for BCR-ABLp. 103
UroVysion Multiprobe FISH in Urinary Cytologyp. 117
Chromogenic In Situ Hybridization in Tumor Pathologyp. 133
Comparative Genomic Hybridization and Fluorescence In Situ Hybridization in Chronic Lymphocytic Leukemiap. 145
Molecular Characterization of Human Papillomaviruses by PCR and In Situ Hybridizationp. 159
A Nested RT-PCR Assay to Detect BCR/ablp. 181
TP53 Mutation Detection by SSCP and Sequencingp. 191
PCR Diagnosis of T-Cell Lymphoma in Paraffin-Embedded Bone Marrow Biopsiesp. 209
Circulating DNA Analysis: Protocols and Clinical Applications Using Taqman Assaysp. 217
Microsatellite Instability: Theory and Methodsp. 237
The Diagnostic and Prognostic Significance of the Methylation Status of Myf-3 in Lymphoproliferative Disordersp. 251
Quantitative Analysis of PRAME for Detection of Minimal Residual Disease in Leukemiap. 267
Determination of Cyclin D1 Expression by Quantitative Real-Time, Reverse-Transcriptase Polymerase Chain Reactionp. 277
Detection of Telomerase hTERT Gene Expression and Its Splice Variants by RT-PCRp. 297
Detection of Telomerase Enzyme Activity by TRAP Assayp. 311
Identification of TP53 Mutations in Human Cancers Using Oligonucleotide Microarraysp. 323
Detection of K-ras Mutations by a Microelectronic DNA Chipp. 337
Microarray-Based CGH in Cancerp. 355
Tissue Microarraysp. 377
Indexp. 391
Table of Contents provided by Rittenhouse. All Rights Reserved.

ISBN: 9781588291608
ISBN-10: 158829160X
Series: Methods in Molecular Medicine
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 393
Published: 15th March 2004
Country of Publication: US
Dimensions (cm): 23.52 x 16.15  x 2.74
Weight (kg): 0.66
Edition Number: 2
Edition Type: Revised