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Microbial Processes and Products : Methods in Biotechnology - Jose Luis Barredo

Microbial Processes and Products

Methods in Biotechnology

By: Jose Luis Barredo (Editor)

Hardcover

Published: 1st February 2005
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The development of biotechnology over the last 20 years, and particularly the use of recombinant DNA techniques, has rapidly expanded the opportu- ties for human benefits from living resources. Efforts to reduce pollution, p- vent environmental damage, combat microbial infection, improve food production, and so on can each involve fermentation or the environmental - lease of microorganisms. Many products of fermentation technology, such as alcoholic beverages, bread, antibiotics, amino acids, vitamins, enzymes, and others, have been influenced by the progress of recombinant DNA techniques. The development of new products or the more efficient manufacturing of those already being produced often involve the use of microorganisms as cell fac- ries for many productions and biotransformations. Microbial Processes and Products is intended to provide practical expe- mental laboratory procedures for a wide range of processes and products me- ated by microorganisms. Although not an exhaustive treatise, it provides a detailed "step-by-step" description of the most recent developments in such applied biotechnological processes. The detailed protocols we provide are cross-referenced in the Notes section, contain critical details, lists of problems and their troubleshooting, as well as safety recommendations that may not n- mally appear in journal articles and can be particularly useful for those un- miliar with specific techniques.

Microbial Processes and Products is the latest in the series Methods in Biotechnology(t), some issues of which have been highly focused and widely relevant (such as Enzymes in Nonaqueous Solvents, Vulfson et al., 2001). This volume (and indeed the entire series) is targeted to the research community. Those researchers finding a relevant chapter in this text are likely to be rewarded. As the series title suggests, this is a 'methods' volume and the sections are packed with detailed protocols, tips and useful practical advice. The text is generally readable and the chapters are well referenced. -Don Cowan, University of the Western Cape "...the sections are packed with detailed protocols, tips, and useful practical advice." - Microbiology Today

Prefacep. v
Contributorsp. xi
Development of Improved Strains and Optimization of Fermentation Processesp. Lei Han
Experimental Design in Microbiologyp. Guillaume E.
Metabolic Engineering of Acremonium chrysogenum to Produce Deacetoxycephalosporin C and Bioconversion to 7-Aminodeacetoxycephalosporanic Acidp. 41
Production of Erythromycin With Saccharopolyspora erythraeap. 65
The Phenylacetyl-CoA Catabolon: Genetic, Biochemical, and Biotechnological Approachesp. 91
Recombinant Microorganisms for the Biosynthesis of Glycosylated Antitumor Compoundsp. 131
Assay Methods for Detection and Quantification of Antimicrobial Metabolites Produced by Streptomyces clavuligerusp. 149
Purification of Plasmid DNA Vectors Produced in Escherichia coli for Gene Therapy and DNA Vaccination Applicationsp. 165
Genome Breeding of an Amino Acid-Producing Corynebacterium glutamicump. 179
Metabolic Activity Profiling by 13C Tracer Experiments and Mass Spectrometry in Corynebacterium glutamicump. 191
Protein and Vitamin Production in Bacillus megateriump. 205
Strategies for Large-Scale Production of Recombinant Proteins in Filamentous Fungip. 225
Strain and Culture Conditions Improvement for ß-Carotene Production With Mucorp. 239
Xanthophylls in Fungi: Metabolic Engineering of the Astaxanthin Biosynthetic Pathway in Xanthophyllomyces dendrorhousp. 257
Methodologies for the Analysis of Fungal Carotenoidsp. 273
Insertional Mutagenesis in the Vitamin B2 Producer Fungus Ashbya gossypiip. 283
Improved Polysaccharide Production Using Strain Improvementp. 301
Use of the Morganella morganii phoC Gene as Reporter in Bacterial and Yeast Hostsp. 313
Gene Expression Arrays in Foodp. 333
Optimization of Proteome Analysis for Wine Yeast Strainsp. 345
Bacteriocin-Producing Strains in a Meat Environmentp. 369
In Vitro and In Vivo Interactions of Nonpathogenic Bacteria With Immunocompetent Cellsp. 381
Volatile Sulfur Detection in Fermented Foodsp. 397
Bioleaching: Analysis of Microbial Communities Dissolving Metal Sulfidesp. 405
Use of PhoN Phosphatase to Remediate Heavy Metalsp. 413
Safe Dispatch and Transport of Biological Materialp. 437
How to Deposit Biological Material for Patent Purposesp. 451
Laws and Regulations for the Protection of Biotechnological Inventionsp. 465
Indexp. 495
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9781588295484
ISBN-10: 1588295486
Series: Methods in Biotechnology
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 516
Published: 1st February 2005
Publisher: Humana Press Inc.
Country of Publication: US
Dimensions (cm): 23.5 x 15.5  x 3.18
Weight (kg): 1.05