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Mhc : Volume 2: A Practical Approach - Nelson Fernandez

Mhc

Volume 2: A Practical Approach

By: Nelson Fernandez (Editor), G. Butcher (Editor)

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MHC2: A Practical Approach offers a comprehensive and accessible guide to the fast-growing and fascinating field of MHC research. This companion volume to MHC1 covers topics including: generation of HLA-specific human monoclonal antibodies; sequencing of proteins; assembly of MHC molecules; expression, purification, and characterization of recombinant soluble MHC class I molecules; mapping the human MHC; derivation and analysis of MHC transgenic mice; cell-surface MHC antigen imaging; sequence analysis of MHC molecules; and techniques to identify the rules governing class II MHC-peptide interaction. This informative guide will be an invaluable aid to clinicians, researchers, and graduate students in the field of immunology.

1: Generation of HLA-specific human monoclonal antibodies 2: Sequencing of proteins isolated by one or two dimensional gel electrophoresis 3: Assembly of MHC molecules: requirements of glycosylation, oxidized glutathione, and peptides 4: Expression, purification, and characterization of recombinant soluble MHC class I molecules 5: The use of pulsed field gel electrophoresis to map the human MHC 6: Derivation and analysis of MHC transgenic mice 7: Imaging of individual cell surface MHC antigens using fluorescent particles 8: Sequence analysis of major histocompatibility molecules 9: Approaches to studying MHC expression in mammals other than primates 10: Techniques to identify the rules governing class II MHC-peptide formation

ISBN: 9780199635559
ISBN-10: 0199635552
Series: Mhc : Book 2
Audience: General
Format: Paperback
Language: English
Number Of Pages: 256
Published: 22nd January 1998
Country of Publication: GB
Dimensions (cm): 23.39 x 15.6  x 1.37
Weight (kg): 0.36