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Methods in Protein Sequence Analysis : The Language of Science - Kazutomo Imahori

Methods in Protein Sequence Analysis

The Language of Science

By: Kazutomo Imahori (Editor), Fumio Sakiyama (Editor)

Hardcover Published: 30th June 1993
ISBN: 9780306444883
Number Of Pages: 310

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The Ninth International Conference on Methods in Protein Sequence Analysis was held for the first time in Asia from September 20 to September 24, 1992 in Otsu (a city near Kyoto), Japan. Approximately 400 delegates attended the meeting. Forty papers were presented orally and 147 poster presentations were discussed. Academic sessions were held from early in the morning until late in the evening. We are confident that the Conference was successful in providing up-to-date information about methods in protein sequence analysis to all participants. Moreover, with the knowledge and understanding of the present standard of various methods of analysis that are being used and will be used, we were able to clarify areas that need to be evaluated, to be improved and be explored further. Major topics in the Conference mostly covered areas in the methodology of protein sequence analysis, such as: micropreparation and microsequencing of proteins, mass spectrometry, post-translational modification, prediction and database analysis, and analysis of protein structures of special interests. The evolution of genetic engineering in molecular biology has greatly accelerated the accumulation of knowledge on the amino acid sequence of novel proteins regardless of whether they are expressed or not expressed in living organisms. In the early stage of accumulation of structural information, the amino acid sequence itself is worthy of notice.

Capillary liquid chromatography: a tool for protein structural analysisp. 3
Routine amino acid sequencing on 2D-gel separated proteins: a protein elution and concentration gel systemp. 13
Capillary electrophoresis in structural characterization of polypeptidesp. 21
Sensitization of gas-phase protein sequencer using fluorescein isothiocyanate (FITC)p. 29
Thiobenzoylation method of protein sequencing: gas chromatography/mass spectrometric detection of 5-acetoxy-2-phenylthiazolesp. 37
Deblocking and subsequent microsequence analysis of N-terminally blocked proteins immobilized on PVDF membranep. 45
Development of novel C-terminal sequencing methodsp. 55
Automated C-terminal sequencing of peptides and proteinsp. 63
A new chemical approach to C-terminal microsequence analysis via the thiohydantoinp. 71
Cyanogen bromide cleavage of proteins on blots and subsequent separation of the fragments by polyacrylamide gel electrophoresis directly from those blotsp. 79
Identification of the disulfide bonds of the human complement component C3p. 87
A novel protease from jack-bean seeds: asparaginyl endopeptidasep. 95
Lysine-specific serine protease from Achromobacter lyticus: its substrate specificity and comparison with trypsinp. 101
Structure and function of snake venom metalloproteinase familyp. 107
Recent advances in protein sequencing by mass spectrometry. Introduction and overviewp. 119
Sequence analysis of peptides presented to the immune system by class I and class II MHC moleculesp. 127
High precision mass spectrometry with the ion trap mass spectrometerp. 135
Electrospray-mass spectrometry, an emerging methodology for elucidating structure-function relationships of proteinsp. 143
A general strategy for the use of mass spectrometric molecular weight information in protein purification and sequence determinationp. 149
Determination of posttranslational modifications by mass spectrometryp. 157
Post-translational modifications of proteinsp. 167
N-Terminal acetylation of mutationally altered form of iso-l-cytochromes c in normal and natl- strains deficient in the major N-terminal acetyl transferase of the yeast Saccharomyces cerevisiaep. 173
Posttranslational glutamylation of several brain tubulin isotypes: structure of the polyglutamyl side chainp. 183
Protein anchoring to membrane by glycosylphosphatidylinositol: Determination of the COOH-terminal signal peptide sequence and GPI-attachment site in bovine liver 5'-nucleotidasep. 191
Pyridylamination for sensitive analysis of sugar chains of glycoproteinsp. 195
Sequence analysis of phosphopeptides and its application for the determination of phosphorylated sites of proteinsp. 199
Prediction of protein structure from multiple sequence alignmentp. 209
Sequence patterns that characterize protein families with a common foldp. 221
Domains and modules of proteinsp. 229
Homology search and prediction of biological function of protein from amino acid sequencesp. 233
Amino acid sequence comparison as an aid to determining evolutionary originsp. 241
Protein databases constructed by quantitative two-dimensional gel electrophoresisp. 247
Biochemistry of a natriuretic peptide familyp. 257
Activin, activin-binding protein (follistatin) and activin receptorp. 261
Do well-populated intermediates represent the pathway of protein folding?p. 269
Alcohol dehydrogenases: patterns of protein evolutionp. 275
Structure and post-translational modification of the lipoyl domain of 2-oxo acid dehydrogenase complexes: a new family of protein domainsp. 283
Structure analysis of the topography and molecular organization of protein-RNA complexes as revealed in ribosomesp. 289
Indexp. 307
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780306444883
ISBN-10: 0306444887
Series: The Language of Science
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 310
Published: 30th June 1993
Publisher: Springer Science+Business Media
Country of Publication: US
Dimensions (cm): 25.4 x 17.8  x 1.91
Weight (kg): 1.87

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