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Methods in Mammary Gland Biology and Breast Cancer Research - Margot M. Ip

Methods in Mammary Gland Biology and Breast Cancer Research

By: Margot M. Ip (Editor), Bonnie B. Asch (Editor)

Paperback Published: 31st December 2000
ISBN: 9780306463976
Number Of Pages: 329

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approaches to the experimental problems that still face us in understanding this most fascinating of organs. Too many people contributed to the completion of this volume to allow acknowledg­ ment of all the individual efforts, but we particularly thank the reviewers whose input into the editorial process was invaluable and the authors of these chapters who revised their text, sometimes more than once, to bring it to the high standards set by the Editors. The Com­ mittee gratefully acknowledges the support ofVysis, Inc. , in the publication of a color figure in Chapter 19, by S. Weber-Hall and Trevor Dale. Finally, we wish to express our heartfelt appreciation to Margot Ip and Bonnie Asch, who worked long and hard to bring this volume to fruition. Margaret C. Neville for the Committee on Mammary Gland Biology Preface One of the most exciting and beneficial developments in research on mammary gland biology and breast cancer has been the influx of increased funding to support this work. This influx, which has been due primarily to the tireless efforts of breast cancer activists to gamer addi­ tional money from various federal and state sources, has led to a rapid expansion of research efforts by attracting numerous new investigators into the field. These new investigators include students, postdoctoral fellows, and scientists from other fields.

In Vivo Model Systems
Mouse Models for Mammary Cancerp. 3
Methods for the Induction of Mammary Carcinogenesis in the Rat Using either 7,12-Dimethylbenz[alpha]anthracene or 1-Methyl-1-Nitrosoureap. 19
A Comparison of the Salient Features of Mouse, Rat, and Human Mammary Tumorigenesisp. 31
Xenograft Models of Human Breast Cancer Lines and of the MCF10AT Model of Human Premalignant, Proliferative Breast Diseasep. 37
Implantation and Characterization of Human Breast Carcinomas in SCID Micep. 51
Special Techniques for In Vivo Studies
The Cleared Mammary Fat Pad and the Transplantation of Mammary Gland Morphological Structures and Cellsp. 67
Preparing Mammary Gland Whole Mounts from Micep. 75
Slow-Release Plastic Pellets (Elvax) for Localized In Situ Treatments of Mouse and Rat Mammary Tissuep. 87
Intramammary Delivery of Hormones, Growth Factors, and Cytokinesp. 97
Hormonal Stimulation of the Mouse Mammary Glandp. 101
In Vitro Model Systems
Collagen Gel Method for the Primary Culture of Mouse Mammary Epitheliump. 111
Chemical-Carcinogen-induced Transformation of Primary Cultures of Mouse Mammary Epithelial Cells Grown Inside Collagen Gelsp. 125
Establishment of Mouse Mammary Cell Linesp. 137
Whole Organ Culture of the Mouse Mammary Glandp. 147
Working with the Mouse Mammary End Budp. 155
Isolation and Culture of Normal Rat Mammary Epithelial Cellsp. 163
Characterization of Normal Human Breast Epithelial Cell Subpopulations Isolated by Fluorescence-Activated Cell Sorting and Their Clonogenic Growth In Vitrop. 177
Isolation and Culture of Human Breast Cancer Cells from Primary Tumors and Metastasesp. 195
Molecular Analysis and Gene Transfer Techniques
mRNA In Situ Hybridization in the Mammary Glandp. 211
Application of In Situ PCR to Studies of the Mammary Glandp. 223
Transfection of Primary Mammary Epithelial Cells by Viral and Nonviral Methodsp. 233
Direct Gene Transfer into the Mammary Epithelium In Situ Using Retroviral Vectorsp. 245
Intraductal Injection into the Mouse Mammary Glandp. 259
Adenoviral and Transgenic Approaches for the Conditional Deletion of Genes from Mammary Tissuep. 271
Transplantation and Tissue Recombination Techniques to Study Mammary Gland Biologyp. 289
Rescue of Mammary Epithelium of Early Lethal Phenotypes by Embryonic Mammary Gland Transplantation as Exemplified with Insulin Receptor Null Micep. 307
Indexp. 317
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780306463976
ISBN-10: 0306463970
Audience: General
Format: Paperback
Language: English
Number Of Pages: 329
Published: 31st December 2000
Country of Publication: US
Dimensions (cm): 25.15 x 19.36  x 2.52
Weight (kg): 0.76

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