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Methods in Enzymology, Volume 419 : Volume 419 - Irina Klimanskaya

Methods in Enzymology, Volume 419

Volume 419

Hardcover

Published: 22nd November 2006
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This is the second of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques focusing on adult stem cells, and written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook" and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using "conventional" and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories.
* Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering
* Presents the latest most innovative technologies
* Addresses therapeutic relevance including FDA compliance and tissue engineering

Ectoderm Isolation and characterization of neural stem cells
Neural stem cells and their manipulation
Retinal stem cells
Epithelial Skin Stem Cells
Dental pulp stem cells
Mesoderm Postnatal skeletal stem cells
Hematopoietic stem cells
Hemangioplast and its progeny
Kidney Epithelial Cells
Ovarian germ cells
Spermatogonial Stem Cells
Endoderm Stem cells in the lung
Pancreatic cells and their progenitors
Intestinal epithelial stem cells and their progenitors
Extraembryonic and perinatal stem cells Trophoblast stem cells
Pluripotent Stem Cells from Germ Cells
Amniotic Fluid and placenta stem cells
Cord blood progenitor cells
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780123736505
ISBN-10: 0123736501
Series: Methods in Enzymology
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 576
Published: 22nd November 2006
Publisher: Elsevier Science Publishing Co Inc
Country of Publication: US
Dimensions (cm): 22.9 x 15.2  x 3.18
Weight (kg): 1.08
Edition Number: 419