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Methods in Enzymology, Volume 415 : Volume 415 - Minoru Fukuda

Methods in Enzymology, Volume 415

Volume 415

Hardcover Published: 1st November 2006
ISBN: 9780121828202
Number Of Pages: 424

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In the past decade, there has been an explosion of progress in understanding the roles of carbohydrates in biological systems. This explosive progress was made with the efforts in determining the roles of carbohydrates in immunology, neurobiology and many other disciplines, examining each unique system and employing new technology. This volume represents the first of three in the Methods in Enzymology series, including Glycomics (vol. 416) and Functional Glycomics (vol. 417), dedicated to disseminating information on methods in determining the biological roles of carbohydrates. These books are designed to provide an introduction of new methods to a large variety of readers who would like to participate in and contribute to the advancement of glycobiology. The methods covered include structural analysis of carbohydrates, biological and chemical synthesis of carbohydrates, expression and determination of ligands for carbohydrate-binding proteins, gene expression profiling including micro array, and generation of gene knockout mice and their phenotype analyses.

N-Glycan processing Non-radioactive analysis of lipid-linked oligosaccharide compositions by fluorophore-assisted carbohydrate electrophoresis (FACE)
Identification of N-glycan-binding proteins for E3 ubiquitin ligases
Familyi 47 aacute;-mannosidases in N-glycan processing
A Cytoplasmic Peptide: N-glycanase
Structural Analysis Glycomic profiling of cells and tissues by mass spectrometry fingerprinting and sequencing methodologies
Structural analysis of sialyl N-glycan using pyridylamination and chromatography followed by multistage tandem mass spectrometry
Determination of glycosylation sites and disulfide bond structures using LC/ESI-MS/MS analysis
Identification of O-GlcNAc sites on proteins
Carbohydrate Synthesis and Antibiotics Chemoenzymatic synthesis of glycan libraries
Glycoconjugate vaccines against Haemophilus influenzae tybe b
Assay of human gastric mucin as a natural antibiotic against Helicobacter pylori
Molecular contacts between antibiotics and the 30S ribosomal particle
Mechanism-based inhibitors to probe transitional states of glycoside hydrolases
Regulating cell surface glycosylation with a small molecule switch
Metabolic labeling of glycans with azido sugars for visualization and glycoproteomics
Carbohydrate Ligand Specificity Functional Proteomic Profiling of Glycan Processing Enzymes
Oligosaccharide Microarrays to Map Interactions of Carbohydrates in Biological Systems
Identification of Ligand Specificities for Glycan-binding Proteins Using Glycan Arrays
High-throughput analysis of lectin-oligosaccharide interactions by automated frontal affinity chromatography
Preparation of Neoglycolipids with Ring-Closed Cores via Chemoselective Oxime-Ligation for Microarray Analysis of Carbohydrate-Protein Interactions
Development of a lectin microarray based on an evanescent-field fluorescence principle
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780121828202
ISBN-10: 0121828204
Series: Methods in Enzymology : Book 415
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 424
Published: 1st November 2006
Publisher: Elsevier Science Publishing Co Inc
Country of Publication: US
Dimensions (cm): 22.9 x 15.2  x 3.18
Weight (kg): 0.95
Edition Number: 415

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