| Contributors to Volume 409 | p. ix |
| Volumes in Series | p. xv |
| Overproduction and Purification of RFC-Related Clamp Loaders and PCNA-Related Clamps from Saccharomyces cerevisiae | p. 1 |
| Functional Assays for Replication Protein A (RPA) | p. 11 |
| Human DNA Ligases I, III, and IV-Purification and New Specific Assays for These Enzymes | p. 39 |
| Enzymatic Mechanism of the WRN Helicase/Nuclease | p. 52 |
| Analysis of the DNA Unwinding Activity of RecQ Family Helicases | p. 86 |
| Characterization of Checkpoint Responses to DNA Damage in Saccharomyces cerevisiae: Basic Protocols | p. 101 |
| Recruitment of ATR-ATRIP, Rad17, and 9-1-1 Complexes to DNA Damage | p. 118 |
| Multiple Approaches to Study S. cerevisiae Rad9, a Prototypical Checkpoint Protein | p. 131 |
| Methods for Studying Adaptation to the DNA Damage Checkpoint in Yeast | p. 150 |
| DNA Damage-Induced Phosphorylation of Rad55 Protein as a Sentinel for DNA Damage Checkpoint Activation in S. cervisiae | p. 166 |
| Methods for Studying Mutagenesis and Checkpoints in Schizosaccharomyces pombe | p. 183 |
| Methods for Determining Spontaneous Mutation Rates | p. 195 |
| Genomic Approaches for Identifying DNA Damage Response Pathways in S. cerevisiae | p. 213 |
| Techniques for [gamma]-H2AX Detection | p. 236 |
| Methods for Studying the Cellular Response to DNA Damage: Influence of the Mre11 Complex on Chromosome Metabolism | p. 251 |
| Detecting Repair Intermediates In Vivo: Effects of DNA Damage Response Genes on Single-Stranded DNA Accumulation at Uncapped Telomeres in Budding Yeast | p. 285 |
| Analysis of Non-B DNA Structure at Chromosomal Sites in the Mammalian Genome | p. 301 |
| Detection and Structural Analysis of R-Loops | p. 316 |
| The Delitto Perfetto Approach to In Vivo Site-Directed Mutagenesis and Chromosome Rearrangements with Synthetic Oligonucleotides in Yeast | p. 329 |
| Assays for Transcriptional Mutagenesis in Active Genes | p. 345 |
| Methods for Studying Chromatin Assembly Coupled to DNA Repair | p. 358 |
| Structure-Function Analysis of SWI2/SNF2 Enzymes | p. 375 |
| Genomic Approach for the Understanding of Dynamic Aspect of Chromosome Behavior | p. 389 |
| Measurement of Chromosomal DNA Single-Strand Breaks and Replication Fork Progression Rates | p. 410 |
| Monitoring DNA Replication Following UV-Induced Damage in Escherichia coli | p. 425 |
| Methods to Study Replication Fork Collapse in Budding Yeast | p. 442 |
| Analysis of Gross-Chromosomal Rearrangements in Saccharomyces cerevisiae | p. 462 |
| Formation and Processing of Stalled Replication Forks-Utility of Two-Dimensional Agarose Gels | p. 477 |
| Poly (ADP-Ribose) Polymerase-1 Activation During DNA Damage and Repair | p. 493 |
| Tyrosyl-DNA Phosphodiesterase (Tdp1) (3'-Phosphotyrosyl DNA Phosphodiesterase) | p. 511 |
| Assaying Double-Strand Break Repair Pathway Choice in Mammalian Cells Using a Targeted Endonuclease or the RAG Recombinase | p. 524 |
| Author Index | p. 541 |
| Subject Index | p. 563 |
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