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Methods In Enzymology, Volume 367 : Volume 367 - Nejat Duzgunes

Methods In Enzymology, Volume 367

Volume 367

Hardcover Published: 27th October 2003
ISBN: 9780121822705
Number Of Pages: 311

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Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies.

*Methods in Liposome Preparation
*Physiochemical Characterization of Liposomes

Contributors to Volume 367p. vii
Prefacep. ix
Volumes in Seriesp. xi
Methods of Liposome Preparation
Extrusion Technique to Generate Liposomes of Defined Sizep. 3
Giant Liposomes in Studies on Membrane Domain Formationp. 15
Preparation and Quantitation of Small Unilamellar Liposomes and Large Unilamellar Reverse-Phase Evaporation Liposomesp. 23
Liposomes Prepared by High Pressure Homogenizersp. 28
Liposome Preparation by Detergent Removalp. 46
Plasmid DNA Vaccines: Entrapment into Liposomes by Dehydration-Rehydrationp. 70
Interdigitation-Fusion Liposomesp. 80
Freeze-Drying of Liposomes: Theory and Practicep. 99
Preparation of Sterile Liposomes by Proliposome-Liposome Methodp. 111
Physicochemical Characterization of Lipsomes
Phase Behavior of Liposomesp. 129
Electrophoretic Characterization of Liposomesp. 148
Viscometric Determination of Axial Ratio of Ellipsoidal DNA-Lipid Complexp. 177
Atomic Force Microscopy Imaging of Liposomesp. 199
Interfacial Properties of Liposomes as Measured by Fluorescent and Optical Probesp. 213
Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence Microscopyp. 233
Determination of Liposome Surface Dielectric Constant and Hydrophobicityp. 253
Enzymatic Assays for Quality Control and Pharmacokinetics of Liposome Formulations: Comparison with Nonnzymatic Conventional Methodologiesp. 272
Author Indexp. 293
Subject Indexp. 305
Table of Contents provided by Ingram. All Rights Reserved.

ISBN: 9780121822705
ISBN-10: 0121822702
Series: Methods in Enzymology : Book 367
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 311
Published: 27th October 2003
Publisher: Elsevier Science Publishing Co Inc
Country of Publication: US
Dimensions (cm): 22.9 x 15.2  x 1.91
Weight (kg): 0.62