Methods In Enzymology, Volume 336

Developmental and Molecular Biological Aspects: Volume 336

By: Ron J. Doyle

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Hardcover | 25 May 2001

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469 Pages

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This volume and its companion, Volume 337, supplement Volume 310, . These volumes provide a contemporary sourcebook for virtually any kind of experimental approach involving biofilms. They cover bioengineering, molecular, genetic, microscopic, chemical, and physical methods.

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Contributors to Volume 336
Preface
Volume in Series
Developmental Processes in Biofilms
Whole Genome DNA Microarray Expression Analysis of Biofilm Development by Vibrio cholerae Ol El Tor	p. 3
Biofilm Formation as a Developmental Process	p. 19
Signaling and Gene Expression in Biofilm
Surface Sensing, Swarmer Cell Differentiation, and Biofilm Development	p. 29
Acylated Homoserine Lactone Detection in Pseudomonas aeruginosa Biofilms by Radiolabel Assay	p. 41
Genetic and Phenotypic Analysis of Multicellular Behavior in Salmonella typhimurium	p. 48
Gene Transfer in Bacterial Biofilms	p. 60
Conversion to Mucoidy in Pseudomonas aeruginosa Infecting Cystic Fibrosis Patients	p. 60
Subtractive Hybridization-Based Identification of Genes Uniquely Expressed or Hyperexpressed during Biofilm Growth	p. 76
Biofilm-Induced Gene Expression and Gene Transfer	p. 84
Directed Movement and Surface-Borne Motility of Myxococcus and Pseudomonas	p. 94
A General Method to Identify Bacterial Genes Regulated by Cell-to-Cell Signaling	p. 102
Genetic and Chemical Tools for Investigating Signaling Processes in Biofilms	p. 108
In Situ Quantification of Gene Transfer Biofilms	p. 129
Transcriptional Analysis of Genes Involved in Pseudomonas aeruginosa Biofilms	p. 144
First Stages of Biofilm Formation: Characterization and Quantification of Bacterial Functions Involved in Colonization Process	p. 152
Growth of Bacteriophage in Bacteria in Biofilms
Phenotype Characterization of Genetically Defined Microorganisms and Growth of Bacteriophase in Biofilms	p. 163
Biofilms of Staphylococci
Methods for Studying Biofilms Produced by Staphylococcus epidermidis	p. 177
Methods to Detect and Analyze Phenotypic Variation in Biofilm-Forming Staphylococci	p. 195
In Vivo Models to Evaluate Adhesion and Biofilm Formation by Staphylococcus epidermidis	p. 206
Genetic and Biochemical Analysis of Staphylococcus epidermidis Biofilm Accumulation	p. 215
In Vitro Methods to Study Staphylococcal Biofilm Formation	p. 239
Efficient RNA Isolation Method for Analysis of Transcription in Sessile Staphylococcus epidermidis Biofilm Cultures	p. 255
Metabolic Potential of Biofilms
Assessment of Metabolic Potential of Biofilm-Associated Bacteria	p. 265
Extracellular Polymers
Characterization of Extracellular Citinolytic Activity in Biofilms	p. 279
Isolation and Biochemical Characterization of Extracellular Polymeric Substances from Pseudomonas aeruginosa	p. 302
Microbiological Aspects of Microbial Biofilm
Approach to Analze Interactions of Microorganisms. Hydrophobic Substrates, and Soil Colloids Leading to Formation of Composite Biofilms, and to Study Initial Events in Microbiogeological Processes	p. 317
Extracellular Polymers of Microbial Communities Colonizing Acient Limestone Monuments	p. 331
Studying Phototrophic and Heterotrophic Microbial Communities on Stone Monuments	p. 340
Identification of Archaea in Objects of Art by Denaturing Gradient Gel Electrophoresis Analysis and Shotgun Cloning	p. 356
Probiotics
Lactobacilli as Vehicles for Targeting Antigens to Mucusal Tissues by Surface Exposition of Foreign Antigens	p. 369
A Dot-Blot Assay for Adhesive Components Relative to Probiotics	p. 389
Understanding Urogenital Biofilms and Potential Impact of Probiotics	p. 403
Surface Characterization and Adhesive Properties of Bifidobacteria	p. 411
Author Index	p. 429
Subject Index	p. 457
Table of Contents provided by Blackwell. All Rights Reserved.

Methods In Enzymology, Volume 336

Developmental and Molecular Biological Aspects: Volume 336

At a Glance

Hardcover

Shipping

How to return your order

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