Methods in Comparative Plant Ecology : A Laboratory Manual

A Laboratory Manual

By: G.A. Hendry (Editor), J.P. Grime (Editor)

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Hardcover | 1 January 1993

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Hardcover
276 Pages

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26.04 x 20.96 x 1.91

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With the growing awareness of the far reaching adverse effects of human economic activity on the biosphere, the methods used to investigate these effects on key plant species are crucial. This book supplies vital information on such methods. The Integrated Screening Programme (ISP) developed by the team of scientists at Sheffield University's Unit of Comparative Plant Ecology has given rise to the classic books "Comparative Plant Ecology" and "The Abridged Comparative Plant Ecology" (now published by Chapman & Hall). This book is a sister publication outlining the methods used by the ISP. Topics covered include: fundamental processes of photosynthesis, respiration, nitrogen and phosphorus metabolism; physiology of water relations; carbon allocation; responses to mineral depletion; day length changes; and chill/freezing, heat stress and other environmental stresses.

Industry Reviews

This book is ring bound which allows the volume to lie open on the laboratory bench. And make no mistake, it will lie open on many laboratory benches! Every plant ecology laboratory will want to have at least one copy. - Journal of Ecology
It will be of value not only to the experienced researcher but also to the novice starting a project...extremely useful - New Phytologists
There is much here of interest to teachers of A level and undergraduate biology and environmental science students. - The Biologist
The manual has been well written and edited...very useful to beginning graduate students who are looking for suitable techniques...You do not have to be an adherent of the comparative plant ecology school to find this book a useful addition to your bookshelf... - Ecology

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Contributors
Preface
Introduction
The need for comparative laboratory studies	p. 1
How to use the manual	p. 5
Abbreviations	p. 7
General procedures and methodologies
Seed collecting, cleaning and long-term storage	p. 9
Seed viability and germination	p. 10
The ISP aerial environment: lighting, day length, temperature and humidity	p. 14
ISP germination and rooting environments; sand, compost and solution culture	p. 19
Harvesting, recording weight, area and length	p. 24
Photographic recording	p. 25
The ISP standard regime	p. 28
Methods of comparative study
Anatomy
Aerenchyma, intercellular gas space and aeration	p. 29
Functional plant anatomy	p. 31
DNA
Chromosome number	p. 36
Nuclear DNA amount	p. 38
Environmental impacts
Anoxia tolerance in germinating seeds, rhizomes, stolons and tubers	p. 41
Response to defoliation	p. 45
Desiccation tolerance	p. 48
Drought tolerance	p. 53
Effect of high temperatures on protein synthesis	p. 55
Herbicide selectivity	p. 58
Plant responses to low temperatures	p. 61
Ozone sensitivity	p. 63
Sulphur dioxide pollution	p. 65
Sunfleck utilization	p. 68
Ultra-violet (UV-B) radiation	p. 72
Growth
Adventitious rooting of cuttings	p. 74
Carbon budget	p. 77
Day length responses	p. 79
Establishment in a vertical light gradient	p. 81
Foraging for light	p. 83
Foraging for mineral nutrients	p. 87
Fresh weight/dry weight and related ratios	p. 91
Constant temperature and shade	p. 92
Fluctuating temperature and drought	p. 95
Mean relative growth rate	p. 98
Response to carbon dioxide enrichment	p. 102
Variation in RGR underlying carbon economy	p. 107
Leaves
Decomposition	p. 110
Defence chemistry	p. 113
Longevity	p. 115
Palatability	p. 116
Tearability	p. 121
Metabolism
Calculation of specific activities of enzymes	p. 123
Carbon isotope discrimination and water use efficiency	p. 125
Nitrate reductase activity	p. 127
Acid phosphatases	p. 129
Photosynthesis and chlorophyll fluorescence: simultaneous measurements	p. 130
Photosynthesis and respiration: use of O[subscript 2] electrodes	p. 136
Respiration: the alternative pathway	p. 140
Respiration: cytochrome c oxidase activity	p. 144
Storage carbohydrates	p. 146
Stress indicators: chlorophylls and carotenoids	p. 148
Stress indicators: electrolyte leakage	p. 152
Stress indicators: lipid peroxidation	p. 154
Mineral nutrients
Chemical analysis	p. 156
Uptake and yield	p. 163
Roots
Architectural analyses	p. 165
Mycorrhizal infection: assessment and quantification	p. 170
Penetration and response to drought	p. 173
Seeds
Buoyancy in air	p. 176
Defence chemistry (cyanide)	p. 178
Defence chemistry (phenols)	p. 180
Dormancy breaking	p. 181
Germination at alternating temperatures	p. 183
Germination at a range of constant temperatures	p. 185
Germination and the high irradiance reaction	p. 187
Germination and soil water potential	p. 190
Mineral nutrient content	p. 192
Morphology and colour	p. 194
Organic reserves	p. 196
Persistence in soil	p. 199
Size	p. 202
Shoots
Architecture and vigour	p. 205
Toxicity
Aluminium	p. 208
Cadmium sensitivity and constitutive resistance	p. 211
Iron	p. 213
Sulphide	p. 215
Variability in seed storage proteins	p. 218
Quantitative synthesis	p. 223
Epilogue: the future development of comparative methodologies	p. 239
The problem	p. 239
The search for an answer	p. 240
Species index	p. 241
Subject index	p. 245
Table of Contents provided by Blackwell. All Rights Reserved.

Methods in Comparative Plant Ecology : A Laboratory Manual

A Laboratory Manual

At a Glance

Hardcover

Industry Reviews

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Defective items

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