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Methods for studying the genetics, molecular biology, physiology, and pathogenesis of the streptococci : Methods in Cell Science - Paula M. Fives-Taylor

Methods for studying the genetics, molecular biology, physiology, and pathogenesis of the streptococci

Methods in Cell Science

By: Paula M. Fives-Taylor (Editor), Donald J. LeBlanc (Editor)

Hardcover Published: 31st July 1999
ISBN: 9780792358152
Number Of Pages: 256

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This book is devoted entirely to methods developed in and for studies of members of the bacterial family Streptococcaceae. Many of the studies that have been conducted on the Streptococcaceae were initiated because of the diseases they cause, or to enhance their utility from an industrial perspective. However, the results of many of these investigations have demonstrated a complexity among some members of the family that warrants an interest in them in their own right, apart from or in addition to any biomedical or industrial considerations.
It is therefore hoped and expected that the advanced methods contained in this book will be of interest to those who work with the streptococci and other Gram-positive organisms, to researchers interested in industrial and medical microbiology and to any researcher who seeks to obtain a better understanding of how microorganisms interact with each other, their environment and their hosts.

Editorial: Methods for studying the genetics, molecular biology, physiology, and pathogenesis of the streptococci
List of contributors
Tn917 transponson mutagenesis and marker rescue of interrupted genes of Streptococcus mutansp. 1
Site-specific homologous recombination mutagenesis in group B streptococcip. 13
Targeted mutagenesis of enterococcal genesp. 21
A lactococcal pWV01-based integration toolbox for bacteriap. 35
Vectors containing streptococcal bacteriophage integrases for site-specific gene insertionp. 51
Streptococcal integration vectors for gene inactivation and cloningp. 59
Induction of transformation in streptococci by synthetic competence stimulating peptidesp. 65
Characterization of the lactococcal conjugative element pRS01 using IS946-mediated mutagenesisp. 71
Use of electroportation in genetic analysis of enterococcal virulencep. 79
Genetic transfer methods for Streptococcus sobrinus and other oral streptococcip. 85
Isolation of enterococcal antigen-encoding genes from genomic librariesp. 95
A simple microtiter plate screening assay for bacterial invasion or adherencep. 107
End-probing: A non-radioactive approach to mapping transponson insertionsp. 113
A method for mapping phage-inducible promoters for use in bacteriophage-triggered defense systemsp. 119
Secretion of heterologous proteins by genetically engineered Streptococcus gordoniip. 127
Examination of streptococcal gene expression in the mammalian environmentp. 137
Analysis of adherence-associated gene expression in Streptococcus parasangusis: A method for RNA isolationp. 143
Development of an integrative, lacZ transcriptional-fusion plasmid vector for Streptococcus mutans and its use to isolate expressed genesp. 153
Use of proteomics and PCR to elucidate changes in protein expression in oral streptococcip. 165
The use of continuous flow bioreactors to explore gene expression and physiology of suspended and adherent populations of oral streptococcip. 181
In vitro systems for investigating group B streptococcal: host cell and extracellular matrix interactionsp. 191
The rat model of endocarditisp. 203
Lipoproteins and other cell-surface associated proteins in streptococcip. 209
Growth of Streptococcal mutans in an iron-limiting mediump. 217
Identification of oral streptococci using PCR-based, reverse-capture, checkerboard hybridizationp. 223
Pulsed-field gel electrophoresis as an epidemiologic tool for enterococci and streptococcip. 233
Cell-based panning as a means to isolate phage display Fabs specific for a bacterial surface proteinp. 241
Subject indexp. 251
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780792358152
ISBN-10: 0792358155
Series: Methods in Cell Science
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 256
Published: 31st July 1999
Publisher: Springer
Country of Publication: NL
Dimensions (cm): 29.21 x 20.96  x 1.27
Weight (kg): 1.04