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Methods for Affinity-Based Separations of Enzymes and Proteins : Progress in Probability - Munishwar N. Gupta

Methods for Affinity-Based Separations of Enzymes and Proteins

Progress in Probability

By: Munishwar N. Gupta (Editor)

Paperback Published: 1st April 2002
ISBN: 9783764363055
Number Of Pages: 225

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Extensive troubleshooting guide Big concerns of biotechnology are either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90 percent of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors. As each chapter includes a succinct introduction, the volume is a handy and instructive reference also for students majoring in these disciplines. The special focus plus the most detailed protocols of up-to-date methods make this book a very useful and unique reference.

List of Contributorsp. VII
Prefacep. IX
Affinity-Based Separation: An Overviewp. 1
Affinity Chromatographyp. 16
Expanded Bed Affinity Chromatographyp. 29
Perfusion Affinity Chromatography for Rapid Antibody Separationsp. 65
Affinity-Based Interactions on Disks for Fast Analysis, Isolation and Conversion of Biomoleculesp. 82
Sugars as Affinity Ligandsp. 115
Macroaffinity Ligands in Bioseparationp. 130
Cellulose-Binding Fusion Proteinsp. 148
Two-Phase Affinity Partitioning of Animal Cells: Implications of Multipoint Interactionsp. 163
Affinity-Based Reverse Micellar Separationsp. 181
Biosensors: An Emerging Tool in Bioanalytical Research - A Kinetic Analysis of the Analyte-Receptor Interactions in Biosensorsp. 195
Guide to Solutionsp. 217
Guide to Enzymesp. 217
Guide to Protocolsp. 217
Troubleshooting Guidep. 218
Indexp. 221
Table of Contents provided by Syndetics. All Rights Reserved.

ISBN: 9783764363055
ISBN-10: 3764363053
Series: Progress in Probability
Audience: General
Format: Paperback
Language: English
Number Of Pages: 225
Published: 1st April 2002
Publisher: Birkhauser Verlag AG
Country of Publication: CH
Dimensions (cm): 25.4 x 17.78  x 1.42
Weight (kg): 0.64