| Production of Artemisinin from in Vitro Cultures of Artemisia annua L. | |
| Introduction | p. 1 |
| In Vitro Approaches | p. 6 |
| Conclusions and Prospects | p. 9 |
| References | p. 10 |
| In Vitro Culture and the Production of Secondary Metabolites in Coriandrum sativum | |
| Introduction | p. 13 |
| Plant Regeneration Via Somatic Embryogenesis | p. 14 |
| Production of Petroselinic Acid from Cell Suspension Cultures | p. 17 |
| Summary and Conclusions | p. 19 |
| Protocol for Plant Regeneration Via Somatic Embryogenesis | p. 19 |
| Protocol for Production of Petroselenic Acid from Cell Suspension Cultures | p. 20 |
| References | p. 21 |
| Polyphenol Production in Crataegus Tissue Cultures | |
| Introduction | p. 23 |
| In Vitro Approaches | p. 28 |
| Oxygen Species Scavenging by Callus Extracts | p. 39 |
| Summary and Conclusion | p. 42 |
| Protocol | p. 43 |
| References | p. 47 |
| Dionaea muscipula Ellis (Venus Flytrap): In Vitro Cultures and in Vitro Production of Secondary Metabolites | |
| Introduction | p. 50 |
| In Vitro Approaches | p. 54 |
| Secondary Metabolites | p. 63 |
| Conclusions and Prospects | p. 68 |
| Protocol | p. 70 |
| References | p. 70 |
| In Vitro Culture and the Production of Secondary Metabolites in Hyoscyamus reticulatus | |
| Introduction | p. 75 |
| In Vitro Approaches | p. 77 |
| Conclusion | p. 91 |
| Protocol | p. 91 |
| References | p. 92 |
| Micropropagation of Hypericum canariense L. for the Production of Hypericin | |
| Introduction | p. 95 |
| Hypericum canariense L | p. 97 |
| In Vitro Culture Studies of Hypericum Species | p. 100 |
| Hypericin from Clonal Propagation of Hypericum canariense L. | p. 105 |
| Future Prospects | p. 107 |
| Conclusion | p. 108 |
| Protocol | p. 108 |
| References | p. 110 |
| Production of Quinolizidine Alkaloids in in Vitro Cultures of Legumes | |
| Introduction | p. 118 |
| Quinolizidine Alkaloid Formation in in Vitro Cultures | p. 123 |
| Why is Quinolizidine Alkaloid Production Reduced in Cell Suspension Cultures? | p. 128 |
| Gene Expression in Suspension-Cultured Cells of L. polyphyllus | p. 129 |
| References | p. 132 |
| Malva sp. (Mallow): In Vitro Culture and the Production of Secondary Metabolites | |
| Introduction | p. 137 |
| The in Vitro Culture of Malva sp | p. 143 |
| Conclusion and Prospects | p. 152 |
| Protocol | p. 152 |
| References | p. 153 |
| In Vitro Culture and the Production of Polyphenol and Volatile Compounds in Ocimum | |
| Introduction | p. 156 |
| In Vitro Studies of Ocimum | p. 157 |
| Tissue Cultures and Phenolic Production of Ocimum | p. 158 |
| Shoot Cultures and Volatile Compounds of Ocimum | p. 168 |
| Conclusion and Prospects | p. 173 |
| Protocol | p. 174 |
| References | p. 176 |
| Pergularia tomentosa in Vitro Culture and the Production of Cardenolides and Other Secondary Metabolites | |
| Introduction | p. 177 |
| In Vitro Culture Studies | p. 181 |
| Cardenolide Production by P. tomentosa Callus and Suspension Cultures | p. 192 |
| Summary and Conclusion | p. 194 |
| Protocol | p. 194 |
| References | p. 196 |
| Indolopyridoquinazolin, Furoquinoline, Canthinone and Protoberbenine-Type Alkaloids from Phellodendron amurense Callus Tissues | |
| Introduction | p. 199 |
| In Vitro Cultures | p. 203 |
| Chemotaxonomy | p. 207 |
| Speculative Biogenesis of the Indolopyridoquinazoline, Furoquinoline, Canthinone and Protoberberine-Type Alkaloids in Callus Tissues | p. 208 |
| Isolation and Characterization of cDNA Clones Encoding Aromatic Amino Acid Decarboxylase During Alkaloid Biosynthesis from P. amurense | p. 209 |
| Time Course Study | p. 211 |
| Productivity of Alkaloids from P. amurense Callus Tissues | p. 213 |
| Summary | p. 214 |
| Protocol | p. 217 |
| References | p. 218 |
| In Vitro Culture and the Production of Secondary Metabolites by Sempervivum spp. | |
| Introduction | p. 222 |
| In Vitro Approaches | p. 227 |
| Clonal Propagation Technique | p. 232 |
| Conclusion and Prospect | p. 235 |
| Protocol | p. 236 |
| References | p. 238 |
| In Vitro Culture and the Production of Steroidal Saponin in Solanum aculeatissimum Jacq. | |
| Introduction | p. 241 |
| In Vitro Approaches | p. 242 |
| Summary and Conclusion | p. 248 |
| Protocol | p. 249 |
| References | p. 250 |
| Solanum chrysotrichum (Schdl.): Distribution, Identification of Antifungal SC-1 Saponin, In Vitro Propagation, Cell Suspension Cultures, Large-Scale Production of SC-1 | |
| Introduction | p. 252 |
| Isolation and Identification of the Antifungal Compound | p. 254 |
| In Vitro Approaches | p. 257 |
| Conclusion and Prospect | p. 265 |
| Protocol | p. 265 |
| References | p. 266 |
| In Vitro Culture and the Production of Secondary Metabolites in Solanum kasianum | |
| Introduction | p. 268 |
| In Vitro Approaches | p. 270 |
| Conclusion and Prospect | p. 277 |
| References | p. 278 |
| In Vitro Culture and the Production of Secondary Metabolites in Stephania | |
| Introduction | p. 281 |
| In Vitro Studies | p. 283 |
| Protocols | p. 299 |
| Summary and Conclusion | p. 303 |
| References | p. 303 |
| Trigonella Species: In Vitro Culture and Production of Secondary Metabolites | |
| Introduction | p. 306 |
| In Vitro Culture Studies | p. 308 |
| Summary and Conclusion | p. 324 |
| References | p. 325 |
| Vaccinium Species (Small-Fruited Berries): In Vitro Culture and the Production of Food Colorants and Phytochemicals | |
| Introduction | p. 328 |
| In Vitro Approaches | p. 333 |
| Summary, Conclusion, and Prospects | p. 341 |
| Protocol | p. 342 |
| References | p. 342 |
| Subject Index | p. 345 |
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