Measuring metabolic rates is central to important questions in many areas of scientific research. Unfortunately these measurements are anything but straightforward, and numerous pitfalls await the novice and even the experienced investigator.
Measuring Metabolic Rates de-mystifies the field, explaining every common variation of metabolic rate measurement, from century-old manometric methods through ingenious syringe-based techniques, direct calorimetry, aquatic respirometry, stable-isotope metabolic measurement and every type of flow-through respirometry. Each variation is described in enough detail to allow it to be applied in practice. Background information on different analyzer and equipment types allows users to choose the best instruments for their application. Respirometry equations - normally a topic of terror and confusion to researchers - are derived and described in enough detail to make their selection and use effortless.
Vital topics such as manual and automated baselining, implementing multi-animal systems, and the correct analysis and presentation of metabolic data are covered in enough detail to turn a respirometry neophyte into a hardened metabolic warrior, ready to take on the task of publication in peer-reviewed journals.
The information is a comprehensive synthesis of current knowledge, bringing together methods used in animal physiology, environmental biophysics and biomechanics into one volume. Indeed, this fulfils the ultimate aim of a biology methods book: to provide a thorough explanation of how the methods should be used, while also explaining why they work and how to interpret results. * British Ecological Society *
Chapter 1: A Brief History of Metabolic Measurement
Chapter 2: Constant Volume and Constant Pressure Respirometry
Chapter 3: Coulometric Respirometry
Chapter 4: Constant Volume Techniques Using Gas Analysis
Chapter 5: Aquatic Oxygen Analysis
Chapter 6: Direct Calorimetry
Chapter 7: Measuring Field Metabolic Rates
Chapter 8: Flowthrough Respirometry: Overview
Chapter 9: Flowthrough Respirometry: The Equations
Chapter 10: Flowthrough Respirometry Using Incurrent Flow Measurement
Chapter 11: Flowthrough Respirometry Using Excurrent Flow Measurement
Chapter 12: Validating Flowthrough Respirometry
Chapter 13: Metabolic Data Analysis and Presentation
Chapter 14: The Varieties Of Gas Analyzers
Chapter 15: The Varieties Of Flow Meters
Chapter 16: The Varieties Of Activity Detectors
Chapter 17: The Varieties Of Scrubbers, Tubing And Tubing Connectors