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Manual of Antisense Methodology : Perspectives in Antisense Science - Gunther Hartmann

Manual of Antisense Methodology

Perspectives in Antisense Science

By: Gunther Hartmann (Editor), Stefan Endres (Editor)

Hardcover

Published: December 2009
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  • Paperback View Product Published: 12th October 2012
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In the past few years, antisense methodology has moved from in vitro studies to in vivo studies and first human trials. While the basic concept of antisense technology is simple, the methodological problems associated with its use are numerous and complex. Antisense- based methods have proven to be a field of research where careful attention to experimental protocols and appropriate controls is necessary. The Manual of Antisense Methodology emphasizes the application of antisense oligonucleotides, and is a guide for the identification of antisense and non-antisense effects in different experimental settings. The work is organized into three sections: antisense application in vitro, antisense application in vivo (animal models) and finally, clinical antisense studies. Where at all possible, the methods are described in sufficient detail to allow reproduction of a given experiment. The Manual of Antisense Methodology will be of interest to researchers in immunology, cancer research, pharmacology and internal medicine; and physicians conducting clinical studies in these fields.

Antisense methodology
Preface
Contributors
The chemistry of antisense oligonucleotides
Chemical synthesis and purification of phosphorothioate antisense oligonucleotidesp. 3
How to choose optimal antisense targets in an mRNAp. 25
Antisense application in vitro
How to characterize and improve oligonucleotide uptake into leukocytesp. 41
Strategies for targeted uptake of antisense oligonucleotides in hepatocytesp. 57
How to exclude immunostimmulatory and other nonantisense effects of antisense oligonucleotidesp. 79
Testing antisense oligonucleotides in controlled celi culture assaysp. 91
Inhibition of TNF synthesis by antisense oligonucleotidesp. 107
Inhibition of IGF-1 receptor expression by antisense oligonucleotides in vitro and in vivop. 125
Antisense application in vivo
How to test antisense oligonucleotides in animalsp. 145
Lipid-based carriers for the systemic delivery of antisense drugsp. 167
Designing a clinical antisense studyp. 191
Indexp. 203
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780792385394
ISBN-10: 079238539X
Series: Perspectives in Antisense Science
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 205
Published: December 2009
Publisher: Springer
Country of Publication: NL
Dimensions (cm): 23.5 x 15.5  x 1.91
Weight (kg): 1.09