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Mammalian Cell Biotechnology : A Practical Approach - M. Butler

Mammalian Cell Biotechnology

A Practical Approach

By: M. Butler (Editor)

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The development of mammalian cell biotechnology has led to an extensive range of commercially-valuable prophylactic, diagnostic and therapeutic compounds which can now be produced routinely by cell culture. This book details the principles and practical techniques upon which this technological development is based. The ten chapters are designed to cover the basic principles and techniques which can be applied to maximize the productivity of cell cultures. Media optimisation and the use of serum-substitutes or growth factors are explained. Methods of genetic manipulation of cells are explored as well as the techniques for maximizing specific protein expression. Chapters are included to explain the design and control of animal cell fermentation processes and the means of extraction of desired products by downstream processing. Finally, a chapter is included to explain the strategies for the application of these techniques to the production of specific compounds which have commercial value. This book will be of value to both the research student of biotechnology in academic institutions and to the industrialist who needs to maximize productivity from mammalian cell processes. The detailed principles and techniques described in the book should advance the understanding and realization of high productivity from mammalian cell cultures. This book is intended for academic and industrial researchers in biotechnology, molecular biology, biochemistry.

'The volume covers a wide range of techniques and protocols and will be of value to laboratory researchers, final year students, and those working in industrial laboratories who wish to raise their productivity' ASLIB Booklist, Vol.56, No.11, November 1991 `. . . good-value basic information in a wide range of topical research areas.' Trends in Biotechnology

M. Butler: The characteristics and growth of cultured cells; A. MacLeod: Serum and its fractionation; N. Jenkins: Growth factors; C. MacDonald: Genetic engineering of animal cells; A. Dickson: Protein expression and processing; C. Harbour & A. Fletcher: Hybridomas: production and selection; A. Handa-Corrigan: Bioreactor design; D. Kilburn: Monitoring and control of bioreactors; A. Lyddiatt: Downstream processing protein recovery; B. Griffiths: Products from animal cells;

ISBN: 9780199632091
ISBN-10: 019963209X
Series: Practical Approach Series
Audience: Professional
Format: Paperback
Language: English
Number Of Pages: 264
Published: 25th July 1991
Country of Publication: GB
Dimensions (cm): 23.2 x 15.5  x 1.5
Weight (kg): 0.47