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Mammalian Artificial Chromosomes : Methods and Protocols - Vittorio Sgaramella

Mammalian Artificial Chromosomes

Methods and Protocols

By: Vittorio Sgaramella (Editor), Sandro Eridani (Editor)

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Published: 30th November 2003
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In 1996, we organized a workshop, inter alia, at the National Research Co- cil in Milan under the generous sponsorship of the European Science Foun- tion. On that occasion, a small group of investigators convened from many countries and presented early evidence of the possibility of assembling basic units of mammalian chromosomes into artificial constructs (or, indeed, red- ing the relevant components to more manageable dimensions and defined c- stitution). Progress in the following years has been slow but steady. Many scientists who took part in the workshop have since been engaged in active and prod- tive research. It goes to the credit of Humana Press to have realized the need for a book on artificial chromosomes that aims to provide better tools to all scientists committed to this field who are confronted with very difficult tech- cal problems. We have strived to cover in Mammalian Artificial Chromosomes: Methods and Protocols all relevant areas of artificial chromosome research, from basic genetics to daring attempts to build new tools for genetic therapy. We are of course grateful to the authors who have accepted the task of describing the technical steps and pitfalls that can be encountered in their research. Rarely has a very delicate methodology been presented with such meticulous care. We have been helped in this enterprise by the excellent librarian of the LITA Institute in Segrate, Italy, Ms. Claudia Piergigli, whom we thank warmly. Ms.

Prefacep. v
Contributorsp. ix
From Natural to Artificial Chromosomes: An Overviewp. 1
Methodologies Applied to Domestic Animal Chromosomesp. 15
Mapping of the Hotspots of Recombination in Human DNA Cloned as Yeast Artificial Chromosomesp. 35
Genomic Imprinting and Its Effects on Genes and Chromosomes in Mammalsp. 53
Centromeres and Neocentromeresp. 77
Identification of Start Sites of Bi-Directional DNA Synthesis at Eukaryotic DNA Replication Originsp. 105
Telomere Length Analysis and In Vitro Telomerase Assayp. 123
Telomerization of Mammalian Cells and Transplantation of Telomerized Cells in Immunodeficient Micep. 147
Chromosome-Based Vectors for Mammalian Cells: An Overviewp. 167
Mammalian Artificial Chromosome Formation in Human Cells After Lipofection of a PAC Precursorp. 187
Use of Natural and Artificial Chromosome Vectors for Animal Transgenesisp. 207
Pronuclear Microinjection of Purified Artificial Chromosomes for Generation of Transgenic Mice: Pick-and-Inject Techniquep. 227
Modification of Human Bacterial Artificial Chromosome Clones for Functional Studies and Therapeutic Applicationsp. 243
Indexp. 267
Table of Contents provided by Ingram. All Rights Reserved.

ISBN: 9781588290960
ISBN-10: 1588290964
Series: Methods in Molecular Biology : Book 240
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 269
Published: 30th November 2003
Publisher: SPRINGER VERLAG GMBH
Country of Publication: US
Dimensions (cm): 23.57 x 15.8  x 2.06
Weight (kg): 0.57