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Low-Temperature Microscopy and Analysis - Patrick Echlin

Low-Temperature Microscopy and Analysis

Hardcover Published: 31st March 1992
ISBN: 9780306439841
Number Of Pages: 540

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The frozen-hydrated specimen is the principal element that unifies the subject of low- temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of com- mon fact and theory upon which their work must be based. Low-Temperature Micros- copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro- molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all the more necessary in electron microscopy because of the enormous increase in resolution that is available with electron-optical instruments, compared to light- optical microscopes.

The Properties and Structure of Waterp. 1
The Structure and Properties of Frozen Water and Aqueous Solutionsp. 19
Sample Cooling Proceduresp. 59
Cryosectioningp. 101
Low-Temperature Fracturing and Freeze-Fracture Replicationp. 141
Freeze-Dryingp. 193
Freeze Substitution and Low-Temperature Embeddingp. 223
Low-Temperature Light Microscopyp. 265
Low-Temperature Transmission Electron Microscopyp. 295
Low-Temperature Scanning Electron Microscopyp. 349
Low-Temperature Microanalysisp. 413
Current Status of Low-Temperature Microscopy and Analysisp. 491
Referencesp. 499
Indexp. 529
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780306439841
ISBN-10: 0306439840
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 540
Published: 31st March 1992
Publisher: Springer Science+Business Media
Country of Publication: US
Dimensions (cm): 26.67 x 18.42  x 3.81
Weight (kg): 1.18