As a result of the development of new methodologies, there has recently been a surge of interest in lipids, and hence lipid analysis, among biochemists and medical scientists. This book covers methods of lipid extraction in solvent and solid phase systems, as well as methods of derivative formation needed for a number of analytical procedures such as gas liquid chromatography and mass spectrometry. It describes in practical detail the techniques for complete analysis of both isolated lipids and lipid mixtures. These include GLC, HPLC, TLC, and NMR. Lipid Analysis: A Practical Approach will be invaluable not only to researchers in lipid biochemistry but also to newcomers in the field from biological, chemical, and ecological backgrounds.
'This is a valuable book for postgraduates and other researchers. It is clear, accurate and reasonably comprehensive ... this blend of detailed practical instructions and lucid explanations of new and developing instrumental techniques is very useful and I am happy to recommend this book.'
B.J.F. Hudson, Chemistry in Britain, August 1993
'This is an excellent book which provides much valuable information for the successful analysis of lipids ... this really is an excellent book which deserves to be widely bought and well used.'
John Hardwood, University of Wales, TIBS 19 - March 1994
'This book gives practical details how to avoid the difficulties and deals with: the chemical nature of lipids; extraction of lipids and derivative formation.'
International Journal of Biochemistry, Vol. 25, No. 12, 1993
F.D. Gunstone: Introduction; S. Hamilton, R.J. Hamilton, & P.A. Sewell: Extraction of lipids and derivative formation; R.J. Henderson, & D.R. Tocher: Thin-layer chromatography; R.P. Evershed: Gas chromatography of lipids; P.A. Sewell: High-performance liquid chromatography; C.G. Taylo: Radiotracers in lipid analysis; F.D. Gunstone: High resolution 1H and 13C NMR; R.P. Evershed: Mass spectrometry of lipids.