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Introduction to Animal Cytogenetics - Herbert C. Macgregor

Introduction to Animal Cytogenetics

Paperback Published: 31st July 1993
ISBN: 9780412546006
Number Of Pages: 238

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"Introduction to Animal Cytogenetics" presents animal cytology as a science of seeing and interpreting chromosome form and behaviour and, above all, of appreciating its evolutionary significance. Its principal objective is to help students develop a basic understanding and confidence on all matters relating to animal chromosomes. Molecular biology aspects are introduced whenever appropriate and helpful. Students should want to understand the developmental and evolutionary significance of molecular mechanisms that form the basis of visible structures and adaptive processes, and will learn more about DNA by seeing it in action than by studying it from the base upwards. Following the main series of chapters, there are two special sections that may provide some insight into the human and clinical sides of cytogenetics and give the student a flavour for what goes on in the field. Medical cytogenetics and cancer cytogenetics are treated with a principles and strategies approach, basically whetting the students' appetites for chromosomal and cellular ways of tackling these very complex and difficult areas of medical sciences. The text is supplemented by comprehensively documented protocols for four simple laboratory practical sessions, each of which has undergone extensive field trials and can be relied on to provide an interesting and challenging learning exercise. The protocols include all the information that is likely to be required by the teacher or laboratory assistant for setting up the practical class and by the student for carrying out the work and evaluating the results.

Industry Reviews

An excellent textbook. - Bioogist

Chromosomes in mitosisp. 1
The cell cyclep. 2
Chromosome numbersp. 3
Chromosome shapep. 4
Chromosome sizep. 6
Chromosomes in mitosisp. 7
DNA per nucleus (C-value)p. 9
Karyology and evolutionp. 13
Historical perspectivep. 13
Chromosome bandingp. 16
Karyotypes, genomes and evolutionp. 21
Meiosisp. 29
Basic principlesp. 29
Timing and sequencep. 30
The synaptonemal complexp. 45
Chromosome pairingp. 48
Chiasmata and crossing overp. 54
Disjunctionp. 58
Duration of meiosisp. 63
Meiosis in rearranged chromosomesp. 64
Sex chromosomes and sex determinationp. 71
Polyploidy in animalsp. 77
The problems of becoming polyploidp. 77
Some specific examplesp. 79
Evolving polyploidyp. 81
Inactivation and elimination of chromosomesp. 85
The Barr bodyp. 85
X inactivation in mice and mulesp. 87
The timing of inactivationp. 87
The role of the second Xp. 88
The genetic and molecular basis of inactivationp. 90
Inactivation and imprintingp. 90
More about insectsp. 95
Ascaris and chromatin eliminationp. 96
The protozoan macronucleusp. 100
DNA replication, endomitosis and trophic polyploidyp. 103
The semiconservative replication of chromosomesp. 103
The principles of trophic polyploidyp. 104
Some examples of polyploid cellsp. 106
Polytene chromosomesp. 110
Selective DNA replicationp. 113
The nucleolus organizer and gene amplificationp. 119
Basic molecular biologyp. 119
Identifying nucleolus organizersp. 121
Rings, Christmas trees and transcription unitsp. 124
Understanding amplificationp. 130
Variation and evolutionp. 134
The activity of nucleolus organizersp. 136
Lampbrush chromosomesp. 139
History and technologyp. 139
Basic organizationp. 143
Two famous hypothesesp. 146
Lampbrushes under the electron microscopep. 147
In situ hybridizationp. 149
The read-through hypothesisp. 152
Three simple experiments with enzymesp. 153
Some more questions about lampbrushesp. 157
DNA sequences and genome evolutionp. 161
The classification of DNA sequencesp. 161
Variation in repeat sequence DNAp. 162
Agents of changep. 164
Sister chromatid exchangep. 166
What keeps genomes in check?p. 169
The story of a liberated chromosomep. 172
Human clinical cytogeneticsp. 177
Conventions for describing human chromosomesp. 177
The problem of aneuploidyp. 181
Trisomy 21 and Down's syndromep. 183
Monosomy Xp. 185
The clinical cytogenetics laboratoryp. 186
Outlook for 2001p. 193
Appendix: A selection of laboratory practical protocolsp. 201
Introductionp. 201
Some notes on light microscopyp. 202
Laboratory practical 1: The mitotic chromosomep. 205
Human chromosome preparations from peripheral bloodp. 205
Nuclear sexing from oral mucosal smearsp. 208
Nuclear sexing from polymorphonuclear leucocytes (neutrophils)p. 209
Information for instructors and technical staffp. 210
Laboratory practical 2: Cell size, DNA content and polyploidyp. 213
Cell size and nuclear DNA contentp. 213
The squash techniquep. 213
Polytene chromosomesp. 215
Information for instructors and technical staffp. 218
Laboratory practical 3: Meiosisp. 221
Locustsp. 221
Newts and salamandersp. 223
Information for instructors and technical staffp. 224
Laboratory practical 4: Lampbrush chromosomesp. 226
Isolation of the nucleusp. 226
Isolation of the chromosomes and nucleolip. 227
Observationp. 228
Information for instructors and technical staffp. 229
Species indexp. 233
Indexp. 235
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780412546006
ISBN-10: 0412546000
Audience: General
Format: Paperback
Language: English
Number Of Pages: 238
Published: 31st July 1993
Publisher: Chapman and Hall
Country of Publication: GB
Dimensions (cm): 23.39 x 15.6  x 1.35
Weight (kg): 0.36