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In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues. Visualization of the location of genes on chromosomes or of specific mRNAs or viruses in tissues is crucial for understanding the organization, regulation, and function of genes. It is a therefore a core technique in all areas of biomedical research. In Situ Hybridization: A Practical Approach 2/e is the second edition of one of the most successful Practical Approach books, published in 1992. Since the first edition was published, a number of important technical advances have been made. The new edition has been thoroughly updated to contain protocols detailing the major techniques of in situ hybridization currently in use: in situ hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and hapten labelled); analysis using light and electron microscopes; whole mount in situ hybridization; double detection of RNAs, and RNA plus protein; and fluorescent in situ hybridization to detect chromosomal sequences. The protocols are complemented by advice on strategies for successful results, descriptions of the theoretical basis of in situ hybridization and important new developments in gene expression databases. The procedures described are widely applicable to many systems. The use of in situ hybridization in PCR is covered in a separate volume: Herrington and O'Leary (Eds) PCR 3 - PCR in situ hybridization: A Practical Approach (OUP, 1997). All the authors have extensive practical experience of establishing reliable techniques of in situ hybridization. This book will be useful to all researchers at all levels who use in situ hybridization.
|Theory and practice of in situ hybridization|
|Oligonucleotide probes for in situ hybridization|
|Detection of mRNA in tissue sections with radiolabelled riboprobes|
|In situ hybridization of mRNA with hapten labelled probes|
|Two color in situ hybridization|
|Electron microscope in situ hybridization: the non-isotopic post-embedding procedure|
|Detection of genomic sequences by fluorescence in situ hybridization|
|Gene expression databases|
|List of suppliers|
|Table of Contents provided by Publisher. All Rights Reserved.|
Series: Practical Approach Series
Number Of Pages: 242
Published: 1st March 1999
Country of Publication: GB
Dimensions (cm): 23.6 x 15.8 x 1.3
Weight (kg): 0.42
Edition Number: 2
Edition Type: Revised