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In Situ Hybridisation : Application to Developmental Biology and Medicine - N. Harris

In Situ Hybridisation

Application to Developmental Biology and Medicine

By: N. Harris (Editor), D. G. Wilkinson (Editor)

Hardcover Published: 31st August 1990
ISBN: 9780521380621
Number Of Pages: 302

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Advances in our understanding of biological mechanisms have frequently been associated with the development of new techniques. In situ hybridisation is a classic case of just such an advance. The technique effectively combines histochemistry with molecular biology and enables the rapid analysis of the distribution of RNA, or DNA, in the tissues. The information gained from this has caused something of a revolution in our understanding of developmental biology, since a fundamental aspect of development is the spatial and temporal expression of genes. In addition the technique has found application in the field of medicine, where it is giving new insights into the functioning of healthy tissues and the diagnosis and study of diseases. This book brings together contributions from leaders in the application of this technique and will help the reader review and help the would-be exponent through the various options and variations of the technique.

Industry Reviews

"...very useful for biologists, molecular biologists, biophysicists, and advanced students." Journal of Steroid Biochemistry and Molecular Biology

Non radio isotopic labels for in situ hybridisation histochemistry: a histochemist+s view
Use of haptenised nucleic acid probes in fluorescent in situ hybridisation
The use of complementary RNA probes for the identification and localisation of peptide messenger RNA in the diffuse neuroendocrine system
Contributions of the spatial analysis of gene expression to the study of sea urchin development
Advantages and limitations of in situ hybridisation as exemplified by the molecular genetic analysis of Drosophila development
The use of in situ hybridisation to study the localisation of maternal mRNAs during Xenopus oogenesis
In situ hybridisation in the analysis of genes with potential roles in mouse embryogenesis
Evolution of algal plasmids from eukaryotic endosymbionts
Localisation of expression of male flower specific genes from maize by in situ hybridisation
Tissue preparation techniques for in situ hybridisation studies of storage-protein gene expression during pea seed development
Investigation of gene expression during plant gametogenesis by in situ hybridisation
Sexing the human conceptus by in situ hybridisation
Nonisotopic in situ hybridisation in human pathology
The demonstration of viral DNA in human tissues by in situ hybridisation
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780521380621
ISBN-10: 0521380626
Series: Cambridge Studies in Management (Hardcover)
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 302
Published: 31st August 1990
Country of Publication: GB
Dimensions (cm): 22.86 x 15.24  x 1.75
Weight (kg): 0.57

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