Immunochemistry is of immense importance to virtually all areas of modern biology and medicine. Immunochemistry I and its companion volume, Immunochemistry II, offer a comprehensive compendium of the latest techniques in immunochemistry in a clear and accessible format. Volume I details the current strategies and methods used in the production of high quality antibodies, including chemically and genetically engineered antibodies, human monoclonal antibodies from B lymphocytes, anti-tumor antibodies, and antibodies packaged in eggs. Other topics include the uses of enzymes and antibodies in immunoassays; the standardization of antigens and antibodies; the latest ELISA techniques including enzyme amplification systems; enzyme immunoassays for detecting cell-surface molecules, single cell products, and proliferation; photoluminescence immunoassays; the incorporation of antibodies and their fragments into immunotoxins; and the use of synthetic peptides in mapping the epitopes of both antibodies and T-cells.
Volume II discusses the measurements of immunologically relevant molecules; immunofluorescence and immunoenzyme histochemistry; the use of synthetic peptides and liposomes in immunology; the use of antibodies as analytical probes and separation tools in fluorescence, blotting, and affinity chromatography; the measurement of antibody affinity; solid-phase assays of avidity; confocal laser scanning microscopy; flow cytofluorimetry; analysis of adhesion molecules; assays for complement receptors and for complement components. Each chapter includes clear technical advice, hints and tips, and sample results to help insure practical success.
"An outstanding "how-to" manual...Well written, concise, and practical as advertised in the title. This book is highly recommended to virtually all scientists toiling in the immunochemical vineyard."--John Robinson, MD, Doody's Journal