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Immunochemical Protocols : Methods in Molecular Biology - Robert Burns

Immunochemical Protocols

Methods in Molecular Biology

By: Robert Burns (Editor)

Hardcover

Published: 13th December 2004
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Immunochemical techniques have been in use for many years with early examples of bacterial strain typing dating back to the 1940s. The basis for the science is the exquisite elegance of the mammalian immune system with its ability to recognize foreign proteins and to manufacture antibody m- ecules that strongly bind to the substances that elicited them. Not only are potentially harmful pathogens and toxins recognized by the immune system, but the system can be persuaded to manufacture antibodies to an astonishing array of substances. In the early days of this science, all antibodies for investigative work were produced by immunizing mammals with the substance of interest, followed by regular donor bleeds that yielded antisera. Serum produced in this way yields heterogenic populations of antibody molecules recognizing different epitopes on the target protein, which may be adequate for its intended p- poses, but can also cause problems of crossreactivity. In 1975, Kohler and Milstein reported that spleen cells from immune donor animals could be immortalized, cloned from single cells, and grown in continuous culture. This original work described the method for the production of monoclonal antibodies.

Reviews of Previous editions:

"...a treasury of detailed information, both within the protocols and in the Notes, which in some chapters are extensive...will be useful to both those who already have some experience in this area and...to those who wish to embark on new techniques."

-Microbiology Today



An excellent and well arranged compilation of a range of antibody techniques....An excellent laboratory manual.-Immunoassay

Immunization Strategies for Antibody Production
Synthetic Peptides as Antigens for Antibody Production
Production of Polyclonal Antibodies in Rabbits
Making Hybridomas
Growing Hybridomas
Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments
Purification of Antibodies and Preparation of Antibody Fragments
Conjugation of Antibodies to Alkaline Phosphatase
Conjugation of Antibodies to Horseradish Peroxidase
Conjugation of Antibodies to Fluorescein or Rhodamine
Conjugation of Antibodies to Biotin
Preparation of Colloidal Gold Probes
Immunogold Probes in Light Microscopy
Immunoelectron Microscope Techniques in Plant Virus Diagnosis
Dual-Antibody Sandwich Enzyme-Linked Immunosorbent Assay
Competitive Enzyme-Linked Immunosorbent Assay
Immunoblotting Techniques
Epitope Mapping
Generation and Testing of Immunoaffinity Columns
Immunocapture-Polymerase Chain Reaction
Magnetic Cell Sorting
Cell Enrichment and Immunochemical Staining
Index
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9781588292742
ISBN-10: 1588292746
Series: Methods in Molecular Biology
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 318
Published: 13th December 2004
Publisher: Humana Press Inc.
Country of Publication: US
Dimensions (cm): 23.5 x 15.5  x 2.54
Weight (kg): 0.64
Edition Number: 3
Edition Type: Revised