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Immunochemical Protocols : Methods in Molecular Biology - Robert Burns

Immunochemical Protocols

Methods in Molecular Biology

By: Robert Burns (Editor)

Hardcover Published: 13th December 2004
ISBN: 9781588292742
Number Of Pages: 318

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This new edition of a much praised classic includes both basic method for producing and using antibodies and advanced techniques required by today's state-of-the art investigators. The basic sections of the book stress the first principles of antibody production and guide the reader through the processes and pitfalls of immunochemistry. The advanced sections focus on specific applications and cover the immunochemical techniques now used in laboratories. New material includes techniques for making new monoclonal cell lines, for producing good quality monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis.

Reviews of Previous editions:
"...a treasury of detailed information, both within the protocols and in the Notes, which in some chapters are extensive...will be useful to both those who already have some experience in this area and...to those who wish to embark on new techniques."
-Microbiology Today

An excellent and well arranged compilation of a range of antibody techniques....An excellent laboratory manual.-Immunoassay

Immunization Strategies for Antibody Production
Synthetic Peptides as Antigens for Antibody Production
Production of Polyclonal Antibodies in Rabbits
Making Hybridomas
Growing Hybridomas
Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments
Purification of Antibodies and Preparation of Antibody Fragments
Conjugation of Antibodies to Alkaline Phosphatase
Conjugation of Antibodies to Horseradish Peroxidase
Conjugation of Antibodies to Fluorescein or Rhodamine
Conjugation of Antibodies to Biotin
Preparation of Colloidal Gold Probes
Immunogold Probes in Light Microscopy
Immunoelectron Microscope Techniques in Plant Virus Diagnosis
Dual-Antibody Sandwich Enzyme-Linked Immunosorbent Assay
Competitive Enzyme-Linked Immunosorbent Assay
Immunoblotting Techniques
Epitope Mapping
Generation and Testing of Immunoaffinity Columns
Immunocapture-Polymerase Chain Reaction
Magnetic Cell Sorting
Cell Enrichment and Immunochemical Staining
Index
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9781588292742
ISBN-10: 1588292746
Series: Methods in Molecular Biology
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 318
Published: 13th December 2004
Publisher: Humana Press Inc.
Country of Publication: US
Dimensions (cm): 23.5 x 15.5  x 2.54
Weight (kg): 0.64
Edition Number: 3
Edition Type: Revised