| Introduction: Seven Blind Men and An Elephant | p. 1 |
| Identification of Genes and Construction of a Transcriptional Map in Xq28 | p. 5 |
| Use of cDNA Selection and Evolutionarily Conserved Sequences to Isolate Transcribed Sequences from Region Xp11.21 | p. 11 |
| Identification of cDNAs by Direct Hybridization Using Cosmid Probes | p. 23 |
| Locus Specific Identification of Transcribed Sequences Using YACs and Whole Yeast Genomic DNA | p. 29 |
| Towards a Transcriptional Map of Human Chromosome 21 | p. 37 |
| Isolation of Expressed Sequences from the Chromosome 17q21 BRCA1 Region by Magnetic Bead Capture | p. 51 |
| Towards a Transcriptional Map of the q21-q22 Region of Chromosome 7 | p. 65 |
| Direct cDNA Selection Using Human and Mouse cDNAs: Application to Xq13.3 Chromosomal Region | p. 81 |
| A Sandwich-Hybridization Method for Specific and Efficient Selection of cDNA Clones from Genomic Regions | p. 91 |
| Novel Strategy for Isolating Unknown Coding Sequences from Genomic DNA by Generating Genomic-cDNA Chimeras | p. 101 |
| Identifying and Directly Purifying Transcribed Elements by Coincident Sequence Cloning | p. 111 |
| Finding Candidate Genes by Preparative in situ Hybridization | p. 123 |
| Direct cDNA Screening of Genomic Reference Libraries - A Rapid Method for the Identification of Transcribed Sequences in Large Genomic Regions | p. 139 |
| Identification of Expressed Sequences on Human Chromosome 9q32-34 | p. 157 |
| An Exon Trapping System Providing Size Selection of Spliced Clones and Facilitating Direct Cloning | p. 169 |
| Isolation of Gene Sequences from the BRCA1 Region of Chromosome 17q21 by Exon Amplification | p. 183 |
| Isolation of Coding Sequence from Cosmids and YACs by Exon Amplification | p. 199 |
| Integrated Transcriptional Maps of Large DNA Regions: Towards a Transcriptional Map of Human Chromosome 21 | p. 213 |
| Shallow Shotgun Sequencing as a Strategy for Finding Coding Exons | p. 229 |
| Requirements in Screening cDNA Libraries for New Genes and Solutions Offered by SBH Technology | p. 239 |
| Establishing Catalogues of Expressed Sequences by Oligonucleotide Fingerprinting of cDNA Libraries | p. 253 |
| PCR-Based Technologies to Study Differential Gene Expression in Rat Brain | p. 261 |
| 259 Human Brain Expressed Sequence Tags (ESTs): Chromosome Localization, Subregional Assignment, and Sequence Analysis | p. 273 |
| Mapping cDNAs by Hybridization to Gridded Arrays of DNA from YAC Clones | p. 289 |
| Discussion | p. 299 |
| Index | p. 303 |
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