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Heterotrimeric G Proteins : Volume 237 - John N. Abelson

Heterotrimeric G Proteins

Volume 237

By: John N. Abelson (Editor), Melvin I. Simon (Editor), Ravi Iyengar (Editor)

Hardcover

Published: 1st June 1994
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This volume of Methods in Enzymology and its companion Volume 238 include molecular, biological, and biochemical methods for the study of cell surface heterotrimeric G proteins (Volume 237) and their effectors (Volume 238). Methods unique to signal transducing G proteins and general techniques applied to the study of G protein systems are covered.

Praise for the Volume "This is another welcome addition to the excellent 'Methods in Enzymology series... The 'Methods in Enzymology series represents the gold-standard, but even in this context the present volume is special. The list of contributors is impressive, the wide coverage of the field fills a much-needed gap... The best book of its kind on the market. This volume therefore represents extremely good value for money, especially given the relatively long shelf-life of methodology books." --NEUROSCIENCE "I suspect my copy will rapidly acquire the battered look common to well-used techniques manuals once I release it to the laboratory." --Patrick J. Casey in TRENDS IN CELL BIOLOGY Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE "Incomparably useful." --ANALYTICAL BIOCHEMISTRY "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY "The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS "If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA "A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY

Ga Subunits
Measurement of Receptor-Stimulated Guanosine 5'-O-((-Thio)triphosphate Binding by G Proteins
Receptor-Stimulated Hydrolysis of Guanosine 5'-Triphosphate in Membrane Preparations
Regulation of G-Protein Activation by Mastoparans and Other Cationic Peptides
Guanosine 5'-O-((-Thio)triphosphate Binding Assay for Solubilized G Proteins
Activation of Cholera Toxin by ADP-Ribosylation Factors: 20-kDa Guanine Nucleotide-Binding Proteins
Pertussis Toxin-Catalyzed ADP-Ribosylation of G Proteins
Synthesis and Use of Radioactive Photoactivatable NAD+ Derivatives as Probes for G-Protein Structure
Photoaffinity Guanosine 5'-Triphosphate Analogs as a Tool for the Study of GTP-Binding Proteins
Preparation of Activated ( Subunits of Gs and Gis: From Erythrocyte to Activated Subunit
Purification and Separation of Closely Related Members of Pertussis Toxin-Substrate G Proteins
Purification of Transducin
Expression of G-Protein a Subunits in Escherichia coli
Synthesis and Applications of Affinity Matrix Containing Immobilized (( Subunits of G Proteins
Purification of Activated and Heterotrimeric Forms of Gq Proteins
Purification of Phospholipase C-Activating G Protein, G11, from Turkey Erythrocytes
Purification of Recombinant Gqa, G11a, and G16a from Sf9 Cells
Expression and Purification of G-Protein a Subunits Using Baculovirus Expression System
Analysis of G-Protein a and (Subunits by in Vitro Translation
Assays for Studying Functional Properties of in Vitro Translated Gsa Subunit
Myristoylation of G- Protein a Subunits
Specificity and Functional Applications of Antipeptide Antisera Which Identify G-Protein a Subunits
Identification of Receptor-Activated G Proteins: Selective Immunoprecipitation of Photolabeled G-Protein a Subunits
Identification of Mutant Forms of G-Protein a Subunits in Human Neoplasia by Polymerase Chain Reaction-Based Techniques
Detection of Mutations and Polymorphisms of the Gsa Subunit Gene by Denaturing Gradient Gel Electrophoresis
Construction of Mutant and Chimeric G-Protein a Subunits
Design of Degenerate Oligonucleotide Primers for Cloning of G-Protein a Subunits
Microinjection of Antisense Oligonucleo
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780121821388
ISBN-10: 0121821382
Series: Methods in Enzymology : Book 237
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 561
Published: 1st June 1994
Country of Publication: US
Dimensions (cm): 22.86 x 15.24  x 3.18
Weight (kg): 0.95