+612 9045 4394
 
CHECKOUT
Hepatitis B and D Protocols : Volume 1: Detection, Genotypes, and Characterization - Robert K. Hamatake

Hepatitis B and D Protocols

Volume 1: Detection, Genotypes, and Characterization

By: Robert K. Hamatake (Editor), Johnson Y. N. Lau (Editor)

Hardcover Published: 28th January 2004
ISBN: 9781588291059
Number Of Pages: 376

Share This Book:

Hardcover

RRP $618.99
$428.75
31%
OFF
or 4 easy payments of $107.19 with Learn more
Ships in 7 to 10 business days

Other Available Editions (Hide)

  • Paperback View Product Published: 9th November 2010
    $192.76

For volume 1: A broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. In this first of two volumes Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. An accompanying second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. For both volumes: A broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. The first volume Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. The second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. The set offers both new and experienced investigators an encyclopedic collection of powerful tools for studying HBV and HDV infections, an essential resource for finding new therapies to treat chronically infected patients.

From the reviews:

"Both volumes provide an excellent reference for background information and detailed experimental investigations for both Hepatitis B and Hepatitis D. A major attribute of the volumes is that as they cover a wide range of subjects, the reader has the opportunity to access information they would not normally encounter. Volumes 1 and 2 are an excellent reference source and the methodologies described present the opportunity for both new and experienced researchers to study the molecular aspects of HBV and HDV infection."-SGM Quarterly

"...an excellent reference for background information and detailed experimental investigations for both Hepatitis B and Hepatitis D...." - Microbiology Today

"The book is an extremely comprehensive collection of valuable modern protocols on detection of HBV DNA in serum and liver ... . In summary, the general layout of the book is of excellent quality. Details are painstakingly provided so that sophisticated readers can profit from first hand top quality information derived from people who have personally worked at the bench. The substantial number of references will also allow easy access to the specific literature in the field." (M. Mondelli, Digestive and Liver Disease, Vol. 37, 2005)

Dedicationp. v
Forewordp. vii
Forewordp. ix
Prefacep. xi
Contributorsp. xvii
Contents for Volume 2p. xxi
Detection of Viral Markers
Overview of Commercial HBV Assay Systemsp. 3
Detection of HBV DNA in Serum Using a PCR-Based Assayp. 15
Detection of HBV DNA by Oligonucleotide Probingp. 21
Detection of HBV RNA in Serum of Patientsp. 29
Quantification of HBV Covalently Closed Circular DNA from Liver Tissue by Real-Time PCRp. 41
In Situ Hybridization for the Detection and Localization of HBV DNA in Liver Sectionsp. 51
Quantitative Assay of Hepatitis B Surface Antigen in Serum or Plasma Using Laurell Electrophoresisp. 57
In Situ Detection of Hepatitis B Viral Antigens: An Immunohistochemical Approachp. 65
Detection of Hepatitis B Virus X Antigen by Immunohistochemistry and Western Blottingp. 71
Detection of Serum HDV RNA by RT-PCRp. 85
Nonradioisotopic In Situ Hybridization for HDV RNAp. 95
Detection and Characterization of Small and Large HDV Antigensp. 99
Immunohistochemical Detection of Hepatitis Delta Antigenp. 107
Viral Genotypes and Variants
HBV Vaccine-Escape Variantsp. 115
HBV Genotyping and Analysis for Unique Mutationsp. 125
A One-Filter-Three-Probe Assay for Defective Interference (DI) Effects of Naturally Occurring Core Internal Deletion (CID) Variants of Human Hepatitis B Virusp. 151
Detection of Hypermodified Middle-Envelope (M) Proteins Secreted from Naturally Occurring HBV Variants Containing a preS2 Internal Deletionp. 165
Hepatitis B Viral Genotyping with the Research INNO-LiPA HBV Genotyping Line Probe Assayp. 175
Molecular Biological Characterization
Processing of Hepatitis B Virus Surface Proteinsp. 189
Binding of Duck Carboxypeptidase D to Duck Hepatitis B Virusp. 199
Nucleoprotein Transport of HBV Capsid Particlesp. 213
Phosphorylation Analysis of Hepatitis B Virus Core Protein in Mammalian Cellsp. 227
Study of HBV Replication Capacity in Relation to Sequence Variation in the Precore and Core Promoter Regionsp. 235
A cis/trans Genetic Test for Pleiotropic Phenotypes Associated with a Frequent Naturally Occurring Mutation at Amino Acid 97 of HBV Core Proteinp. 247
Studying DHBV Polymerase by In Vitro Transcription and Translationp. 259
Expression and Purification of Functional Hepatitis B Virus Polymerase in the Baculovirus Insect Cell Systemp. 271
Localization of Duck Hepatitis B Virus Polymerase Within Cellsp. 281
Endogenous Polymerase Assay for the Analysis of Hepatitis B Virus in Transgenic Micep. 295
Transcriptional Control of Hepatitis B Virusp. 303
In Vitro Reconstitution of [varepsilon]-Dependent Duck Hepatitis B Virus Replication Initiationp. 315
Hepatitis B Viruses: A Triple Threat for Malignant Transformation of Hepatocytesp. 327
Indexp. 339
Table of Contents provided by Rittenhouse. All Rights Reserved.

ISBN: 9781588291059
ISBN-10: 1588291057
Series: Methods in Molecular Medicine : Book 1
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 376
Published: 28th January 2004
Publisher: Humana Press Inc.
Country of Publication: US
Dimensions (cm): 22.9 x 15.2  x 1.91
Weight (kg): 0.73