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Hemoglobins : Hemoglobins, Part B: Biochemical and Analytical Methods Biochemical and Analytical Methods: Part B Volume 231 - John N. Abelson

Hemoglobins

Hemoglobins, Part B: Biochemical and Analytical Methods Biochemical and Analytical Methods: Part B Volume 231

By: John N. Abelson (Editor), Melvin I. Simon (Editor), Johannes Everse (Editor), Kim D. Vandegriff (Editor), Robert M. Winslow (Editor)

Hardcover Published: 30th March 1994
ISBN: 9780121821326
Number Of Pages: 743

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Hemoglobin has been involved in the most significant advances in our understanding of modern genetics and molecular biology. Now, hemoblobin is again central to a new area: development of artificial blood (blood substitute.) This volume of Methods in Enzymology and its companion, Volume 232, will be indispensable to anyone with a serious interest in this emerging field. They completely update and extend the information presented in Volume 76 published a decade ago.

Key Features
* Preparation, purification, and analysis of human hemoglobin and of various non-human hemoglobins
* Preparation of hemoglobin hybrids and of model heme compounds
* Preparation of chemically modified hemoglobins and of recombinant hemoglobins
* Analytical procedures presented for studying hemoglobin stability and degradation and for the formation of xenobiotic adducts of hemoglobin

Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

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"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY

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"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS

"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA

"A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY

Preparation, Purification, and Analysis of Human Hemoglobin
Pilot-Scale Preparation of Hemoglobin Solutions
Detection of Red Cell Membrane Components in Human Hemoglobin Preparations
Detection of Globin Chains by Reversed-Phase High-Performance Liquid Chromatography
Structural Characterization of Hemoglobin Variants Using Capillary Electrophoresis and Fast Atom Bombardment Mass Spectrometry
Purification of Glycated Hemoglobin
Measurement of Endotoxin Levels in Hemoglobin Preparations
Preparation and Characterization of Nonhuman Hemoglobins
Preparation and Characterization of Insect Hemoglobins from Chironomus thummi thummi
Preparation and Characterization of Invertebrate Globin Complexes
Fish Hemoglobins
Conformational and Functional Characteristics of Bovine Hemoglobin
Hemoglobin Hybrids and Model Hemes
Synthetic Hemes
Semisynthesis of Hemoglobin
Preparation of Mixed Metal Hybrids
Chemically Modified Hemoglobins
Preparation of Hemoglobin Derivatives Selectively or Randomly Modified at Amino Groups
Amidation of Basic Carboxyl Groups of Hemoglobin
Bis(pyridoxal) Polyphosphates as Specific Intramolecular Cross-Linking Agents for Hemoglobin
Preparation of Intramolecularly Cross-Linked Hemoglobins
Derivatives of Hemoglobin Prepared by Reaction with Aryl or Alkyl Isothiocyanates
Hemoglobin Polymerization
Preparation of Conjugated Hemoglobins
Structural Characterization of Modified Hemoglobins
Recombinant Hemoglobin
Production of Human Hemoglobin in Escherichia coli Using Cleavable Fusion Protein Expression Vector
Expression of Recombinant Human Hemoglobin in Escherichia coli
Production of Recombinant Human Hemoglobin A in Saccharomyces cerevisiae
Purification and Characterization of Recombinant Human Sickle Cell Hemoglobin Expressed in Yeast
Preparation of Recombinant Hemoglobin in Transgenic Mice
Transgenic Swine as Recombinant Production System for Human Hemoglobin
Hemoglobin Stability and Degradation
Detection, Formation, and Relevance of Hemichromes and Hemochromes
Measuring Relative Rates of Hemoglobin Oxidation and Denaturation.
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780121821326
ISBN-10: 0121821323
Series: Methods in Enzymology : Book 231
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 743
Published: 30th March 1994
Publisher: ACADEMIC PR INC
Country of Publication: US
Dimensions (cm): 22.86 x 15.24  x 4.14
Weight (kg): 1.19

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