| Introduction | p. 1 |
| Isolation and Enumeraton of Anaerobic and Microaerophilic Bacteria in Aquatic Habitats | p. 9 |
| Isolation and Cultivation of Hyperthermophilic Bacteria from Marine and Freshwater Habitats | p. 21 |
| Isolation of Psychrophilic Bacteria | p. 31 |
| Isolation and Characterization of Bacteriocytes from a Bivalve-Sulfur Bacterium Symbiosis | p. 33 |
| General Techniques for the Isolation and Culture of Marine Protists from Estuarine, Littoral, Psammolittoral, and Sublittoral Waters | p. 41 |
| Long-Term Culture of Marine Benthic Protists | p. 51 |
| Behavior and Bioenergetics of Anaerobic and Microaerobic Protists | p. 59 |
| Culturing Free-Living Marine Phagotrophic Dinoflagellates | p. 67 |
| Enrichment, Isolation, and Culture of Free-Living Heterotrophic Flagellates | p. 77 |
| Determination of Pressure Effects on Flagellates Isolated from Surface Waters | p. 91 |
| Isolation, Cloning, and Axenic Cultivation of Marine Ciliates | p. 97 |
| Isolation and Laboratory Culture of Marine Oligotrichous Ciliates | p. 103 |
| Extraction of Protists in Aquatic Sediments via Density Gradient Centrifugation | p. 109 |
| Statistical Analysis of Direct Counts of Microbial Abundance | p. 117 |
| Enumeration and Isolation of Viruses | p. 121 |
| Total Count of Viruses in Aquatic Environments | p. 135 |
| Improved Sample Preparation for Enumeration of Aggregated Aquatic Substrate Bacteria | p. 139 |
| Direct Estimates of Bacterial Numbers in Seawater Samples Without Incurring Cell Loss Due to Sample Storage | p. 143 |
| Total and Specific Bacterial Counts by Simultaneous Staining with DAPI and Fluorochrome-Labeled Antibodies | p. 149 |
| Use of RFLPs for the Comparison of Marine Cyanobacteria | p. 155 |
| Use of High-Resolution Flow Cytometry to Determine the Activity and Distribution of Aquatic Bacteria | p. 163 |
| Phytoplankton Analysis Using Flow Cytometry | p. 175 |
| Enumeration of Phototrophic Pieoplankton by Autofluorescence Microscopy | p. 187 |
| Estimating Cell Concentration and Biomass of Autotrophic Plankton Using Microscopy | p. 199 |
| Preservation and Storage of Samples for Enumeration of Heterotrophic Protists | p. 207 |
| Staining of Heterotrophic Protists for Visualization via Epifluorescence Microscopy | p. 213 |
| A Quantitative Protargol Stain (QPS) for Ciliates and Other Protists | p. 229 |
| Preparation of Pelagic Protists for Electron Microscopy | p. 241 |
| A Rapid Technique for the Taxonomy of Methanogenic Bacteria: Comparison of the Methylreductase Subunits | p. 253 |
| Extraction of DNA from Soils and Sediments | p. 261 |
| Detecting Gene Sequences Using the Polymerase Chain Reaction | p. 267 |
| Quantitative Description of Microbial Communities Using Lipid Analysis | p. 271 |
| Single Cell Identification Using Fluorescently Labeled, Ribosomal RNA-Specific Probes | p. 285 |
| Immunofluorescence Method for the Detection and Characterization of Marine Microbes | p. 295 |
| The Relationship Between Biomass and Volume of Bacteria | p. 303 |
| Microscope Methods for Measuring Bacterial Biovolume: Epifluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy | p. 309 |
| Measurement of Carbon and Nitrogen Biomass and Biovolume from Naturally Derived Marine Bacterioplankton | p. 319 |
| Use of Color Image Analysis and Epifluorescence Microscopy to Measure Plankton Biomass | p. 327 |
| Determination of Size and Morphology of Aquatic Bacteria by Automated Image Analysis | p. 339 |
| Analysis of Microbial Lipids to Determine Biomass and Detect the Response of Sedimentary Microorganisms to Disturbance | p. 347 |
| Total Microbial Biomass Estimation Derived from the Measurement of Particulate Adenosine-5'-Triphosphate | p. 359 |
| Microphytobenthic Biomass Measurement Using HPLC and Conventional Pigment Analysis | p. 369 |
| Microphotometric Analysis of the Spectral Absorption and Fluorescence of Individual Phytoplankton Cells and Detrital Matter | p. 377 |
| Measurement of Elemental Content and Dry Weight of Single Cells: X-Ray Microanalysis | p. 387 |
| Microautoradiographic Detection of Microbial Activity | p. 397 |
| [superscript 14]C Tracer Method for Measuring Microbial Activity in Deep-Sea Sediments | p. 405 |
| Evaluating Bacterial Activity from Cell-Specific Ribosomal RNA Content Measured with Oligonucleotide Probes | p. 415 |
| Use of Fluorogenic Model Substrates for Extracellular Enzyme Activity (EEA) Measurement of Bacteria | p. 423 |
| Photoassimilation of Acetate by Algae | p. 433 |
| Starvation-Survival Strategies in Bacteria | p. 441 |
| Community Respiration Measurements Using a Pulsed O[subscript 2] Electrode | p. 447 |
| Sediment Community Production and Respiration Measurements: The Use of Microelectrodes and Bell Jars | p. 455 |
| Distinguishing Bacterial from Nonbacterial Decomposition of Spartina alterniflora by Respirometry | p. 465 |
| Microbial RNA and DNA Synthesis Derived from the Assimilation of [2,[superscript 3]H]-Adenine | p. 471 |
| Adenosine Triphosphate (ATP) and Total Adenine Nucleotide (TAN) Pool Turnover Rates as Measures of Energy Flux and Specific Growth Rate in Natural Populations of Microorganisms | p. 483 |
| Estimating Production of Heterotrophic Bacterioplankton via Incorporation of Tritiated Thymidine | p. 495 |
| Thymidine Incorporation into DNA as an Estimate of Sediment Bacterial Production | p. 505 |
| Leucine Incorporation as a Measure of Biomass Production by Heterotrophic Bacteria | p. 509 |
| Estimating Conversion Factors for the Thymidine and Leucine Methods for Measuring Bacterial Production | p. 513 |
| Bacterial Production in Anaerobic Water Columns | p. 519 |
| Production of Heterotrophic Bacteria Inhabiting Marine Snow | p. 531 |
| Bacterial Growth Rates Measured by Pulse Labeling | p. 537 |
| Utilization of Amino Acids and Precursors for Amino Acid De Novo Synthesis by Planktonic Bacteria | p. 547 |
| Dialysis Bag Incubation as a Nonradiolabeling Technique to Estimate Bacterioplankton Production In Situ | p. 553 |
| Growth Rates of Natural Populations of Heterotrophic Nanoplankton | p. 557 |
| The Labeled Chlorophyll a Technique for Determining Photoautotrophic Carbon-Specific Growth Rates and Carbon Biomass | p. 563 |
| Incorporation of [superscript 14]CO[subscript 2] into Protein as an Estimate of Phytoplankton N-Assimilation and Relative Growth Rate | p. 573 |
| Membrane-Containing Fungal Mass and Fungal Specific Growth Rate in Natural Samples | p. 579 |
| Radiotracer Approaches for the Study of Plant Polymer Biodegradation | p. 589 |
| Estimating Degradation Rates of Chitin in Aquatic Samples | p. 597 |
| Measurement of Dimethylsulfide (DMS) and Dimethylsulfoniopropionate (DMSP) in Seawater and Estimation of DMS Turnover Rates | p. 601 |
| Sulfate Assimilation by Aquatic Microorganisms | p. 611 |
| Determination of Nitrogenase Activity in Aquatic Samples Using the Acetylene Reduction Procedure | p. 621 |
| Denitrification and Nitrification Rates in Aquatic Sediments | p. 633 |
| Turnover of [superscript 15]NH[subscript 4]+ Tracer in Sediments | p. 643 |
| Microbial Cycling of Inorganic and Organic Phosphorus in the Water Column | p. 649 |
| Approaches for Measuring Stable Carbon and Nitrogen Isotopes in Bacteria | p. 663 |
| Bacterial Sinking Losses | p. 677 |
| Methods for the Observation and Use in Feeding Experiments of Microbial Exopolymers | p. 685 |
| Protistan Grazing Rates via Uptake of Fluorescently Labeled Prey | p. 695 |
| Grazing Rate of Bacterioplankton via Turnover of Genetically Marked Minicells | p. 703 |
| Estimating Rates of Growth and Grazing Mortality of Phytoplankton by the Dilution Method | p. 715 |
| Consumption of Protozoa by Copepods Feeding on Natural Microplankton Assemblages | p. 723 |
| Predation on Planktonic Protists Assessed by Immunochemical Assays | p. 731 |
| Absorption of Microbes by Benthic Macrofauna by the [superscript 14]C.[superscript .51]Cr Dual-Labeling Method | p. 739 |
| Radioisotope Technique to Quantify In Situ Microbivory by Meiofauna in Sediments | p. 745 |
| Index | p. 757 |
| Table of Contents provided by Blackwell. All Rights Reserved. |
