This book is a step-by-step guide to studying growth factors, from the initial search for and characterization of new activities, to sophisticated in vivo analysis and molecular biology approaches such as DNA cloning. It also covers the detection and study of growth inhibitory factors. Growth Factors: A Practical Approach is an invaluable practical guide for both those entering growth factor research for the first time and more experienced researchers. As well as covering a wide variety of techniques, this book includes a valuable appendix covering all known growth factors.
'for anyone planning to tackle these techniques ... this book is an invaluable source of hard-earned practical knowledge and experience from the front'
Louis C. Mahadevan, King's College, Trends in Cell Biology, Vol. 3, December 1993
''this book is strongly recommended to anyone involved in growth-factor research'
C. Jane Robinson, National Institute for Biological Standards and Control, Potters Bar, TIBTECH, March 1994 (Vol. 12)
1: I.A. McKay: Types of growth factor activity: detection and characterization of new growth factor activities
2: E.J.J. Van Zoelen, B. Lelaey, N. Van der Burg, and D. Huylebroeck: Detection of polypeptide growth factors: application of specific bio-assays and PCR technology
3: A.N. Corps, and K.D. Brown: Induction of DNA synthesis and cell division in quiescent cells in response to small peptide growth factors
4: P. Sanjeeva Reddy and B.H. Cochran: Novel gene transcription in response to growth factors: gene isolation by differential plaque hybridization
5: J.M.W. Slack: Embryological assays for growth factors
6: R.T. Ballock and A.B. Roberts: The role of TGF- in bone growth and bone repair
7: R.J. Akhurst: Localization of growth factor mRNA in tissue sections by in-situ hybridization
8: P. Robinson, P. Whitehead, and W. Hume: Detection and characterization of growth inhibitory factors: epidermal inhibitory pentapeptide (EIP)
9: T.J. Dudgeon, J.M. Clements, and M.G. Hunter: Expression of growth factors in microbial systems
10: S.J. Capper: Immunoassays for growth factors
11: C. Linge and M.R. Green: Determination of growth factor receptor distribution by immunocytochemistry or autoradiography and quantitation by ligand binding or FACS analysis
Series: Practical Approach Series
Number Of Pages: 272
Published: 3rd April 1993
Country of Publication: GB
Dimensions (cm): 23.39 x 15.6
Weight (kg): 0.47