This Volume contains the papers presented by twenty-eight invited speakers at the symposium entitled, "Genetic Manipulation of Woody Plants," held at Michigan State University, East Lansing, Michigan, from June 21-25, 1987. Also included are abstracts of contributed poster papers presented during the meeting. That the molecular biology of woody plants is a rapidly expanding field is attested to by the large attendance and high level of enthusiasm generated at the conference. Leading scientists from throughout the world discussed challenging problems and presented new insights into the devel opment of in vitro culture systems, techniques for DNA analysis and manipulation, gene vector systems, and experimental systems that will lead to a clearer understanding of gene expression and regulation for woody plant species. The presence at the conference of both invited speakers and other scientists who work with nonwoody plant species also added depth to the discussions and applicability of the information presented at the conference. The editors want to commend the speakers for their well-organized and informative talks, and feel particularly indebted to the late Dr. Alexander Hollaender and others on the planning committee who assist ed in the selection of the invited speakers. The committee consisted of David Burger (University of California, Davis), Don J. Durzan (University of California, Davis) , Bruce Haissig (U. S. Department of Agriculture Forest Service), Stanley Krugman (U. S. Department of Agriculture Forest Service) , Ralph Mott (North Carolina State University), Otto Schwarz (Univer.sity of Tennessee, Knoxville), and Roger Timmis (Weyerhaeuser Company).
Keynote Address.- Biotechnology Applied to the Improvement of Underground Systems of Woody Plants.- Tissue Culture Systems.- Gene Transfer in Forest Trees.- In Vitro Manipulation of Slash Pine (Pinus elliottii).- Potential Application of Haploid Cultures of Tree Species.- Biochemical and Anatomical Studies of Birch (Betula pendula Roth) Buds Exposed to Different Climatic Conditions in Relation to Growth In Vitro.- Physiological Genetics of Organogenesis In Vitro.- Tissue Culture of Conifers Using Loblolly Pine as a Model.- Use of Leaf Petioles of Hedera helix to Study Regulation of Adventitious Root Initiation.- Nodule Culture: A Developmental Pathway with High Potential for Regeneration, Automated Micropropagation, and Plant Metabolite Production from Woody Plants.- Physiology of Bud Induction in Conifers In Vitro.- Use of Protoplasts and Cell Cultures for Physiological and Genetic Studies of Conifers.- Plantlet Regeneration In Vitro Via Adventitious Buds and Somatic Embryos in Norway Spruce (Picea abies).- Dna Analysis and Manipulation.- Analysis of Host Range in Transformation of Higher Plants by Agrobacterium tumefaciens.- Development of a DNA Transfer System for Pines.- Development of Glyphosate-Tolerant Populus Plants through Expression of a Mutant aroA Gene from Salmonella typhimurium.- Inheritance and Evolution of Conifer Organelle Genomes.- DNA Analysis During Growth and Development.- The Anaerobic Stress Response and its Use for Studying Gene Expression in Conifers.- Regulation of Gene Expression.- Metabolic Phenotypes in Somatic Embryogenesis and Polyembryogenesis.- Alkaloid Production from Cinchona Cell and Organ Systems.- Biosynthesis of Lower Terpenoids: Genetic and Physiological Controls in Woody Plants.- Approaches to Altering Regulatory Controls of Secondary Pathways in Cultured Cells.- Tissue Cultures of Euphorbia Species.- Control of Morphogenesis in Calocedrus decurrens Tissue Culture.- Intregration of Genetic Manipulation into Breeding Programs.- Potential of Cell Culture in Plantation Forestry Programs.- Multiplication of Meristematic Tissue: A New Tissue Culture System for Radiata Pine.- Integrating Biotechnology into Woody Plant Breeding Programs.- Contents: Poster Abstracts.- Poster Abstracts.- Roster of Speakers, Participants, and Personnel.